Pikulev A T, Shcherban' A I
Vopr Med Khim. 1979 Nov-Dec;25(6):719-23.
Effect of noradrenaline on activity of Na, K-ATPase from heavy microsomes of rat brain was studied in vitro; the effect of noradrenaline was shown to be dose dependent. The maximal activation of the enzyme was observed at 5.10(-5) M concentration of the catecholamine; an increase of its concentration above 5.10(-4) M inhibited Na, K-ATPase. beta-Adrenolytic drug propranolol /1.10(-5) M/ did not affect the noradrenaline action. At the same time, an alpha-adrenolytic drug phentholamine /1.10(-5) M/ decreased down to zero the activating effect of the catecholamine, but did not affect the inhibitory effect of noradrenaline at high concentrations /1.10(-3) M/. Effects of selective alpha-adrenomimetic compound phenylephrine were also studied. The data obtained suggest that the activating effect of noradrenaline on Na, K-ATPase from rat brain is mediated through alpha-adrenoreceptors.
体外研究了去甲肾上腺素对大鼠脑重微粒体中钠钾 - ATP酶活性的影响；结果表明去甲肾上腺素的作用呈剂量依赖性。在儿茶酚胺浓度为5×10⁻⁵ M时观察到该酶的最大激活；当其浓度增加到5×10⁻⁴ M以上时，会抑制钠钾 - ATP酶。β - 肾上腺素能阻断药普萘洛尔（1×10⁻⁵ M）不影响去甲肾上腺素的作用。同时，α - 肾上腺素能阻断药酚妥拉明（1×10⁻⁵ M）可使儿茶酚胺的激活作用降至零，但不影响高浓度（1×10⁻³ M）去甲肾上腺素的抑制作用。还研究了选择性α - 肾上腺素能激动剂苯肾上腺素的作用。所得数据表明，去甲肾上腺素对大鼠脑钠钾 - ATP酶的激活作用是通过α - 肾上腺素能受体介导的。
