Elaev N R, Andreev V P, Shavrina N V
Biull Eksp Biol Med. 1983 Feb;95(2):40-2.
Preincubation of rat brain homogenates with acetylcholine (ACh) in concentrations of 10(-3)-10(-5) M for 60 minutes produces an essential increment (15-30%) in activity of microsomal Na, K-ATPase. Analogous effect was exerted by the acetylcholinesterase inhibitor eserine (10(-5)-10(-6) M). Acetylcholine has no effect in the presence of actinomycin D. Dialysis of microsomes isolated from the homogenate incubated with ACh leads to a decrease in the enzyme activity and release to the dialysate of low-molecular factor activating Na, K-ATPase of intact microsomes. The latter fact evidences the ACh-induced synthesis of activating factor and inhibition of Na, K-ATPase synthesis. After the animals are administered eserine (0.2-0.4 mg/kg), isolated microsomes show a reduced level of Na, K-ATPase (by 10-15%). Dialysis of microsomes leads to an appreciable elevation (by approximately 40%) of the enzyme activity and release into the dialysate of the inhibitory factor. The differences in the effects of eserine in vivo and in vitro suggest that during the impairment of brain integrity certain effects are excluded from the processes of the control over Na, K-ATPase activity. One of these may involve the impairment of intercellular interactions, for example, the disappearance of the effect on cholinoceptive cells of internuncial neurons that release inhibitory neurotransmitters (catecholamines).
将大鼠脑匀浆与浓度为10⁻³ - 10⁻⁵ M的乙酰胆碱（ACh）预孵育60分钟，可使微粒体Na⁺,K⁺ - ATP酶活性显著增加（15% - 30%）。乙酰胆碱酯酶抑制剂毒扁豆碱（10⁻⁵ - 10⁻⁶ M）也有类似作用。在放线菌素D存在的情况下，乙酰胆碱无作用。对用ACh孵育过的匀浆中分离出的微粒体进行透析，会导致酶活性降低，并向透析液中释放能激活完整微粒体Na⁺,K⁺ - ATP酶的低分子因子。后一事实证明了ACh诱导激活因子的合成以及对Na⁺,K⁺ - ATP酶合成的抑制。给动物注射毒扁豆碱（0.2 - 0.4 mg/kg）后，分离出的微粒体显示Na⁺,K⁺ - ATP酶水平降低（10% - 15%）。对微粒体进行透析会使酶活性显著升高（约40%），并向透析液中释放抑制因子。毒扁豆碱在体内和体外作用效果的差异表明，在脑完整性受损期间，某些对Na⁺,K⁺ - ATP酶活性控制过程的影响被排除。其中之一可能涉及细胞间相互作用的受损，例如，释放抑制性神经递质（儿茶酚胺）的中间神经元对胆碱能感受细胞的作用消失。
