Lei Junping, Gu Xiaosong, Ye Zhong, Shi Jianbo, Zheng Xing
1Department of Cardiovascular Diseases, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Clin Appl Thromb Hemost. 2014 Mar;20(2):212-8. doi: 10.1177/1076029612458967. Epub 2012 Sep 9.
The anti-inflammatory, antioxidative, and antiarteriosclerosis activities of simvastatin along with its protective effects on the endothelium suggest that it may also have antiaging effects. The aim of this study was to investigate the antiaging effects of simvastatin as well as its effects on sirtuin 1 (SIRT1) expression in endothelial cells. Aged rats and human umbilical vein endothelial cells were treated with simvastatin in the presence and absence of oxidized low-density lipoprotein (OX-LDL). Aortic β-galactosidase staining was undertaken to determine senescence, and SIRT1 protein expression was evaluated using Western blot analysis. After simvastatin therapy, arterial endothelial cell aging was significantly reduced, and SIRT1 expression was significantly increased. The OX-LDL significantly accelerated the senescence of umbilical vein endothelial cells and decreased SIRT1 expression. The OX-LDL-induced downregulation of SIRT1 was blocked by simvastatin. Simvastatin treatment also reduced umbilical vein endothelial cell aging and increased SIRT1 expression.
辛伐他汀的抗炎、抗氧化和抗动脉粥样硬化活性及其对内皮的保护作用表明它可能也具有抗衰老作用。本研究的目的是探讨辛伐他汀的抗衰老作用及其对内皮细胞中沉默调节蛋白1（SIRT1）表达的影响。在存在和不存在氧化型低密度脂蛋白（OX-LDL）的情况下，用辛伐他汀处理老年大鼠和人脐静脉内皮细胞。进行主动脉β-半乳糖苷酶染色以确定衰老，并使用蛋白质印迹分析评估SIRT1蛋白表达。辛伐他汀治疗后，动脉内皮细胞衰老显著减轻，SIRT1表达显著增加。OX-LDL显著加速脐静脉内皮细胞衰老并降低SIRT1表达。辛伐他汀可阻断OX-LDL诱导的SIRT1下调。辛伐他汀治疗还可减轻脐静脉内皮细胞衰老并增加SIRT1表达。
