Johnson Michael A, Henderson Beric R
Westmead Institute for Cancer Research; University of Sydney; Westmead Millennium Institute at Westmead Hospital; Westmead, Australia.
Bioarchitecture. 2012 Jul-Aug;2(4):138-42. doi: 10.4161/bioa.21182. Epub 2012 Jul 1.
IQGAP1 is an important cytoskeletal regulator, known to act at the plasma membrane to bundle and cap actin filaments, and to tether the cortical actin meshwork to microtubules via plus-end binding proteins. Here we describe the novel subcellular localization of IQGAP1 at the cytoplasmic face of the nuclear envelope, where it co-located with F-actin. The IQGAP1 and F-actin staining overlapped that of microtubules at the nuclear envelope, revealing a pattern strikingly similar to that observed at the plasma membrane. In detergent-extracted cells IQGAP1 was retained at cytoskeletal structures at the nuclear envelope. This finding has new implications for involvement of IQGAP1 in cell polarization and migration events and potentially in cell cycle-associated nuclear envelope assembly/disassembly.
IQGAP1是一种重要的细胞骨架调节因子，已知其在质膜上发挥作用，使肌动蛋白丝成束并加帽，并通过正端结合蛋白将皮质肌动蛋白网络与微管相连。在此，我们描述了IQGAP1在核膜细胞质面的新亚细胞定位，它与F-肌动蛋白共定位。IQGAP1和F-肌动蛋白的染色与核膜处的微管染色重叠，揭示出一种与在质膜处观察到的模式极为相似的模式。在经去污剂处理的细胞中，IQGAP1保留在核膜处的细胞骨架结构上。这一发现对于IQGAP1参与细胞极化和迁移事件以及潜在地参与细胞周期相关的核膜组装/解体具有新的意义。
