New Haven, Conn. From Plastic and Reconstructive Surgery and Biomedical Engineering, Yale University School of Medicine.
Plast Reconstr Surg. 2013 Jan;131(1):27-37. doi: 10.1097/PRS.0b013e3182729cfc.
Adipose-derived mesenchymal stem cells are a robust, multipotent cell source. They are easily harvested and exhibit promise in a variety of regenerative applications. The purpose of this study was to evaluate the aging impact on adipose-derived mesenchymal stem cells, relating to morphology, senescent properties, growth factor expression, and osteogenesis.
Cells obtained from distinct age groups (infant, adult, and elderly) were cultured. Morphology was examined using microscopy, and cell surface markers were interrogated using flow cytometry. Telomere length was measured using real-time polymerase chain reaction. Expression of pertinent angiogenic and osteogenic growth factors was compared. Osteogenic capability was investigated further by evaluating induction response, and by quantification of mRNA expression of RUNX-2 and osteocalcin.
The same isolating ratio of mesenchymal stem cells was derived from each donor, regardless of age. The infant adipose-derived stem cells exhibited elongated spindle morphology and increased telomere length compared with older cells. Angiogenic factors were more highly expressed by infant cells, whereas osteogenic expression was similar among all ages. Response to osteogenic induction was more profound in infant than in older stem cells, as evidenced by alkaline phosphatase and alizarin red staining, as was bone-related gene expression.
Adipose-derived mesenchymal stem cells are available across all age groups. Infant-derived cells are morphologically spindle-shaped, with long telomeres, and exhibit enhanced angiogenic and osteogenic capabilities compared with older cells. Conversely, all age groups exhibit similar osteogenic paracrine activity, and the authors posit that clinical applicability is conserved during the adult to elderly period.
脂肪间充质干细胞是一种强大的、多能的细胞来源。它们易于采集，并在多种再生应用中具有广阔的应用前景。本研究旨在评估脂肪间充质干细胞的衰老影响，包括形态学、衰老特性、生长因子表达和成骨作用。
从不同年龄组（婴儿、成年和老年）获得的细胞进行培养。使用显微镜观察形态，使用流式细胞术检测细胞表面标志物。使用实时聚合酶链反应测量端粒长度。比较相关血管生成和成骨生长因子的表达。通过评估诱导反应和 RUNX-2 和骨钙素 mRNA 表达的定量，进一步研究成骨能力。
无论年龄大小，从每个供体中提取的间充质干细胞的分离比例相同。与老年细胞相比，婴儿脂肪源性干细胞表现出更长的梭形形态和更长的端粒。婴儿细胞表达更高水平的血管生成因子，而所有年龄组的成骨表达相似。碱性磷酸酶和茜素红染色表明，婴儿比老年干细胞对成骨诱导的反应更为明显，骨相关基因的表达也是如此。
脂肪间充质干细胞可在所有年龄组中获得。与老年细胞相比，婴儿来源的细胞呈梭形形态，具有较长的端粒，并且表现出增强的血管生成和成骨能力。相反，所有年龄组均表现出相似的成骨旁分泌活性，作者推测在成年到老年期间，临床适用性得以保持。
