Department of Oncology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China.
Mol Med Rep. 2012 Dec;6(6):1351-4. doi: 10.3892/mmr.2012.1074. Epub 2012 Sep 10.
Radiotherapy is the principal therapy for nasopharyngeal carcinoma (NPC) at early stages. A number of chemotherapeutic methods have been used to inhibit the progression of NPC at elevated stages. Suramin has been reported to inhibit the growth of certain tumor cells via various pathways. In the present study, we aimed to analyze the effects of suramin on the proliferation of NPC cells (CNE-2). Suramin was proved to demonstrate NPC cell growth-inhibiting effects both in a dose- and time-dependent manner. To determine the potential mechanisms of these effects, western blotting and flow cytometric analysis were performed. Suramin was found to have the potential to induce cell cycle arrest in S-phase CNE-2 cells. Additionally, we found that the OPN level may decrease in suramin-treated CNE-2 cells. The changes of certain apoptosis- and p-AKT-associated proteins possibly regulating the OPN expression were measured by western blotting. In conclusion, suramin may function as a potential agent for the adjunctive therapy of NPC.
放射疗法是早期鼻咽癌（NPC）的主要治疗方法。许多化疗方法已被用于抑制晚期 NPC 的进展。苏拉明已被报道通过多种途径抑制某些肿瘤细胞的生长。在本研究中，我们旨在分析苏拉明对 NPC 细胞（CNE-2）增殖的影响。苏拉明证明以剂量和时间依赖的方式显示 NPC 细胞生长抑制作用。为了确定这些作用的潜在机制，进行了 Western blot 和流式细胞术分析。苏拉明有可能诱导 S 期 CNE-2 细胞的细胞周期停滞。此外，我们发现 OPN 水平在苏拉明处理的 CNE-2 细胞中可能降低。通过 Western blot 测量了可能调节 OPN 表达的某些凋亡和 p-AKT 相关蛋白的变化。总之，苏拉明可能作为 NPC 辅助治疗的潜在药物。
