Orhan Yahya T, Karagözlü Cem, Sarioğlu Sülen, Yilmaz Osman, Murat Nergiz, Gıdener Sedef
Department of Pathology, Dokuz Eylül University, School of Medicine, İzmir, Turkey.
Turk J Gastroenterol. 2012 Aug;23(4):333-8. doi: 10.4318/tjg.2012.0343.
BACKGROUND/AIMS: The effect of kefir on peptic ulcer disease was evaluated in an experimental model, with non-steroid anti-inflammatory drugs, together with the determination of gastric mucus secretion by quantitative digital histochemistry.
The experimental group included 28 male albino Wistar rats. After a diet with standard rat bait for 7 days, 14 rats were fed with kefir for 7 days while the others were kept on the same diet. At the 14th day, indomethacin was injected to 7 of the rats fed on kefir and to 7 of the rats on standard rat bait. All the rats were sacrificed after 4 hours. Gastric erosion and ulceration were scored histopathologically. Mucosal mucus was quantified by image analysis, and periodic acid-Schiff stained area percentage was determined.
Erosion and ulceration were identified only in cases that received indomethacin. In the cases on kefir, erosion was identified in 6 cases (86%) and ulceration in 1 case. Rats fed on standard diet had erosion in 4 cases (57%) and ulceration in 3 (43%), but the difference was statistically insignificant (Mann-Whitney test, p=0.25). The stained area percentage for gastric mucus was not different between the four groups (Kruskal-Wallis test, p=0.313).
These findings suggest that kefir does not change gastric mucus secretion. Although statistically insignificant, as there were more cases with ulceration in cases on the rat diet, kefir might have a beneficial effect on peptic ulcer disease induced by non-steroid anti-inflammatory drug. This requires further evaluation in larger series.
背景/目的：在一个实验模型中，使用非甾体抗炎药评估了开菲尔对消化性溃疡疾病的影响，并通过定量数字组织化学测定胃黏液分泌情况。
实验组包括28只雄性白化Wistar大鼠。在用标准鼠饵喂养7天后，14只大鼠用开菲尔喂养7天，而其他大鼠继续相同饮食。在第14天，给7只食用开菲尔的大鼠和7只食用标准鼠饵的大鼠注射吲哚美辛。4小时后处死所有大鼠。通过组织病理学对胃糜烂和溃疡进行评分。通过图像分析对黏膜黏液进行定量，并测定过碘酸-希夫染色面积百分比。
仅在接受吲哚美辛的病例中发现糜烂和溃疡。在食用开菲尔的病例中，6例（86%）发现糜烂，1例发现溃疡。食用标准饮食的大鼠中，4例（57%）有糜烂，3例（43%）有溃疡，但差异无统计学意义（曼-惠特尼检验，p = 0.25）。四组之间胃黏液的染色面积百分比没有差异（克鲁斯卡尔-沃利斯检验，p = 0.313）。
这些发现表明开菲尔不会改变胃黏液分泌。虽然差异无统计学意义，但由于食用大鼠饮食的病例中溃疡病例更多，开菲尔可能对非甾体抗炎药引起的消化性溃疡疾病有有益作用。这需要在更大规模的系列研究中进一步评估。
