七氟醚暴露于 7 日龄大鼠影响神经发生、神经退行性变和神经认知功能。
Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function.
机构信息
Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
出版信息
Neurosci Bull. 2012 Oct;28(5):499-508. doi: 10.1007/s12264-012-1260-4. Epub 2012 Sep 11.
Abstract
OBJECTIVE
Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior.
METHODS
We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia.
RESULTS
Sevoflurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.
CONCLUSION
Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application.
摘要
目的
七氟醚广泛应用于小儿麻醉，先前的研究表明其可导致发育中大脑的神经退行性变。本研究旨在探讨七氟醚对神经发生、神经退行性变和行为的影响。
方法
我们在新生后 7 天的大鼠给予七氟醚 4 小时期间和之后，分别在给药前、给药期间和给药后给予 5-溴脱氧尿苷（S 期标记物），以评估齿状回祖细胞的增殖和氟罗丹明染色的变性。麻醉后 2 周和 6 周进行空间参考记忆测试。
结果
七氟醚减少祖细胞增殖并增加细胞死亡，至少持续至麻醉后 4 天。2 周后空间参考记忆不受影响，但在七氟醚给药后 6 周受到影响。
结论
七氟醚减少发育中大脑的神经发生并增加祖细胞的死亡。这可能介导了七氟醚应用后的迟发性神经认知结果。
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