Division of Clinical Laboratory, Tottori University Hospital, 36-1, Nishi-cho, Yonago, Tottori, Japan.
J Infect Chemother. 2013 Apr;19(2):333-7. doi: 10.1007/s10156-012-0471-y. Epub 2012 Sep 12.
We describe a case of bloodstream infection (BSI) caused by Campylobacter lari in a 58-year-old man diagnosed with lumbar pyogenic spondylitis. Anaerobic blood cultures, taken on the day of admission and on hospital day 4, were positive after 30 h of incubation, although no bacteria were detected by Gram staining. After subculture on 5 % sheep blood agar for 2 days at 35 °C in a 5 % CO2 environment, capnophilic, curved, gram-negative bacteria were recovered. The bacteria were identified as C. lari using a combination of phenotypic identification methods and partial 16S rRNA gene sequencing. The BSI was eradicated following combination therapy with intravenous tazobactam/piperacillin, oral erythromycin, and sulfamethoxazole/trimethoprim. These results suggest that accurate identification, to the species level, is important to determine effective treatment of BSI caused by Campylobacter spp. and can help us to understand the epidemiology.
我们描述了一例由弯曲杆菌属引起的血流感染（BSI）病例，该患者为 58 岁男性，患有腰椎化脓性脊柱炎。入院当天和入院第 4 天采集的厌氧血培养，在孵育 30 小时后呈阳性，尽管革兰氏染色未检测到细菌。在 35°C 5%CO2 环境下于 5%绵羊血琼脂上孵育 2 天后，回收了嗜二氧化碳、弯曲、革兰氏阴性细菌。通过表型鉴定方法和部分 16S rRNA 基因测序的组合，鉴定该细菌为弯曲杆菌属。BSI 在静脉滴注他唑巴坦/哌拉西林、口服红霉素和磺胺甲恶唑/甲氧苄啶联合治疗后得到根除。这些结果表明，准确鉴定到种水平对于确定弯曲杆菌属引起的 BSI 的有效治疗非常重要，并有助于我们了解流行病学。
