MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Macromol Biosci. 2012 Oct;12(10):1436-42. doi: 10.1002/mabi.201200191. Epub 2012 Sep 10.
Annealing of PDADMAC/PSS multilayer microcapsules assembled on PSS-doped CaCO(3) particles at 80 °C for 30 min reduces their size dramatically from 6.9 ± 0.3 to 3.1 ± 0.5 µm. Methylene blue molecules are encapsulated by spontaneous deposition and post-annealing with a concentration of 22 mg · mL(-1), which is 1000 times higher than the feeding value. The unreleased MB molecules are retained stably for a long time, which are then protected by the capsules against reductive enzymes and keep their photodynamic activity. The viability of HeLa cells incubated with the MB-loaded capsules decreases sharply from ≈ 75 (dark cytotoxicity) to ≈ 20% after irradiation with a laser at 671 nm and 60 J · cm(-2) for 75 s.
在 80°C 下退火 30 分钟，将 PDADMAC/PSS 多层微胶囊组装在 PSS 掺杂的 CaCO3 颗粒上，其粒径从 6.9 ± 0.3 µm 急剧减小至 3.1 ± 0.5 µm。亚甲蓝分子通过自发沉积和退火后被封装，浓度为 22 mg·mL-1，是进料值的 1000 倍。未释放的 MB 分子长时间稳定保留，然后被胶囊保护，免受还原酶的影响，并保持其光动力活性。用 671nm 激光照射 60J·cm-2 并持续 75s 后，与负载 MB 的胶囊孵育的 HeLa 细胞的存活率从约 75%（黑暗细胞毒性）急剧下降至约 20%。
