Matsuyama Masahiro, Wakui Masatoshi, Monnai Makoto, Mizushima Tomoko, Nishime Chiyoko, Kawai Kenji, Ohmura Mitsuyo, Suemizu Hiroshi, Hishiki Takako, Suematsu Makoto, Murata Mitsuru, Chijiwa Tsuyoshi, Furukawa Daisuke, Ogoshi Kyoji, Makuuchi Hiroyasu, Nakamura Masato
Department of Surgery, Tokai University School of Medicine, Isehara.
Oncol Lett. 2010 May;1(3):431-436. doi: 10.3892/ol_00000076. Epub 2010 May 1.
Liver metastases of colorectal cancers significantly affect the prognoses of patients. To further understand the biological aspects of the metastatic phenotypes, we established the highly liver-metastatic human colorectal cancer cell subline SW48LM2. The subline was established through the serial intrasplenic transfer of cells derived from poor but visible hepatic tumor foci formed by parental SW48 cells and transferred to NOD/SCID/IL-2Rγc(null) mice. The growth, both under monolayer culture conditions and during the formation of subcutaneous tumors, was similar between the two cell lines, although there were morphological differences in the in vitro spheroid formation. Of 41 molecules reportedly associated positively or negatively with tumor progression, four were overexpressed and four were underexpressed in SW48LM2 cells. Notably, this liver-metastatic cell subline exhibited a strongly reduced expression of the ecto-5'-nucleotidase CD73 as well as an altered metabolism of purine nucleotides. Previous studies showed a positive correlation between CD73 expression and metastatic cancer phenotypes. A reduced CD73 expression in tumor cells, however, may contribute to obtaining insight into the mechanisms of liver metastases.
结直肠癌肝转移显著影响患者的预后。为了进一步了解转移表型的生物学特性，我们建立了高肝转移潜能的人结直肠癌细胞亚系SW48LM2。该亚系是通过将亲代SW48细胞形成的微小但可见的肝肿瘤灶中的细胞，连续脾内接种到NOD/SCID/IL-2Rγc(null)小鼠体内而建立的。尽管在体外球形形成方面存在形态学差异，但两种细胞系在单层培养条件下以及皮下肿瘤形成过程中的生长情况相似。在据报道与肿瘤进展呈正相关或负相关的41种分子中，有4种在SW48LM2细胞中过表达，4种低表达。值得注意的是，这种肝转移细胞亚系表现出ecto-5'-核苷酸酶CD73的表达显著降低以及嘌呤核苷酸代谢改变。先前的研究表明CD73表达与转移性癌症表型之间存在正相关。然而，肿瘤细胞中CD73表达的降低可能有助于深入了解肝转移的机制。
