Rodermond Hans M, Cate Rosemarie Ten, Haveman Jaap, VAN Kuilenburg André, Medema Jan Paul, VAN Bree Chris, Franken Nicolaas A P
Laboratory for Experimental Oncology and Radiobiology (LEXOR), Department of Radiation Oncology, Center for Experimental Molecular Medicine, University of Amsterdam, 1100 DE Amsterdam, The Netherlands.
Oncol Lett. 2010 May;1(3):537-540. doi: 10.3892/ol_00000095. Epub 2010 May 1.
The search for agents that enhance the effect of ionizing radiation has been an object of study for decades. In this study, the sensitizing properties of cyclopentenylcytosine (CPEC) on radiation and cisplatin-induced radiosensitization in human squamous lung carcinoma cells were investigated. Human lung tumour SW-1573 cells (SWp, parental; SWg, gemcitabine-resistant) were incubated with CPEC and cisplatin and subsequently irradiated with different doses of γ-rays. Clonogenic survival was determined to measure the effectiveness of the treatments. CPEC (1 or 2 μM) treatment for 4 h decreased the plating efficiency to 75 and 50% in SWp and SWg cells, respectively. In the SWg cells, 0.1 and 1 μM CPEC for 4 h enhanced the cell killing effect of cisplatin. However, an increase was not noted in the SWp cells. Due to the moderate toxicity of 1 μM for 4 h, this CPEC dose was used in the radiosensitization experiments. However, CPEC neither radiosensitized the lung tumour cells nor enhanced the radiosensitizing effect of cisplatin. A 2-h incubation with 4 μM cisplatin also decreased the plating efficiency to 75-80% in the two cell lines. Using this cisplatin dose, radiosensitization was obtained in the two cell lines. Although cisplatin treatment clearly radiosensitized the lung tumour cells, CPEC treatment did not. Cisplatin-induced radiosensitization was also not enhanced by CPEC.
数十年来，寻找能增强电离辐射效果的药物一直是研究的课题。在本研究中，研究了环戊烯基胞嘧啶（CPEC）对人肺鳞状癌细胞辐射和顺铂诱导的放射增敏作用的敏化特性。将人肺肿瘤SW - 1573细胞（SWp，亲本；SWg，吉西他滨耐药）与CPEC和顺铂一起孵育，随后用不同剂量的γ射线照射。通过测定克隆形成存活率来衡量治疗效果。用CPEC（1或2 μM）处理4小时后，SWp和SWg细胞的接种效率分别降至75%和50%。在SWg细胞中，0.1和1 μM CPEC处理4小时可增强顺铂的细胞杀伤作用。然而，SWp细胞中未观察到增加。由于1 μM处理4小时具有中等毒性，因此该CPEC剂量用于放射增敏实验。然而，CPEC既未使肺肿瘤细胞产生放射增敏作用，也未增强顺铂的放射增敏效果。用4 μM顺铂孵育2小时也使两种细胞系的接种效率降至75 - 80%。使用该顺铂剂量，在两种细胞系中均获得了放射增敏效果。虽然顺铂处理明显使肺肿瘤细胞产生了放射增敏作用，但CPEC处理却没有。CPEC也未增强顺铂诱导的放射增敏作用。
