Tydén E, Löfgren M, Hakhverdyan M, Tjälve H, Larsson P
Division of Pathology, Pharmacology and Toxicology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
J Vet Pharmacol Ther. 2013 Aug;36(4):370-5. doi: 10.1111/jvp.12012. Epub 2012 Sep 12.
In the present study, we examined the gene expression of cytochrome P450 3A (CYP3A) isoenzymes in the tracheal and bronchial mucosa and in the lung of equines using TaqMan probes. The results show that all seven CYP3A isoforms identified in the equine genome, that is, CYP3A89, CYP3A93, CYP3A94, CYP3A95, CYP3A96, CYP3A97 and CYP3A129, are expressed in the airways of the investigated horses. Though in previous studies, CYP3A129 was found to be absent in equine intestinal mucosa and liver, this CYP3A isoform is expressed in the airways of horses. The gene expression of the CYP3A isoenzymes varied considerably between the individual horses studied. However, in most of the horses CYP3A89, CYP3A93, CYP3A96, CYP3A97 and CYP3A129 were expressed to a high extent, while CYP3A94 and CYP3A95 were expressed to a low extent in the different parts of the airways. The CYP3A isoenzymes present in the airways may play a role in the metabolic degradation of inhaled xenobiotics. In some instances, the metabolism may, however, result in bioactivation of the xenobiotics and subsequent tissue injury.
在本研究中，我们使用TaqMan探针检测了马的气管和支气管黏膜以及肺中细胞色素P450 3A（CYP3A）同工酶的基因表达。结果表明，在马基因组中鉴定出的所有七种CYP3A亚型，即CYP3A89、CYP3A93、CYP3A94、CYP3A95、CYP3A96、CYP3A97和CYP3A129，在所研究马匹的气道中均有表达。尽管在先前的研究中发现CYP3A129在马的肠黏膜和肝脏中不存在，但这种CYP3A亚型在马的气道中表达。所研究的个体马匹之间，CYP3A同工酶的基因表达差异很大。然而，在大多数马匹中，CYP3A89、CYP3A93、CYP3A96、CYP3A97和CYP3A129在气道的不同部位高表达，而CYP3A94和CYP3A95低表达。气道中存在的CYP3A同工酶可能在吸入的外源性物质的代谢降解中起作用。然而，在某些情况下，这种代谢可能导致外源性物质的生物活化及随后的组织损伤。
