气动辅助电喷雾离子淌度谱法用于完整蛋白质的定量分析。
Pneumatically assisted electrospray-ion mobility spectrometry for quantitative analysis of intact proteins.
机构信息
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
出版信息
Talanta. 2012 Sep 15;99:29-35. doi: 10.1016/j.talanta.2012.05.015. Epub 2012 May 17.
In this work, quantitative analysis of intact proteins using ion mobility spectrometry (IMS) is introduced. For this purpose a pneumatically assisted electrospray ionization source with a new design was constructed. Liquid and nebulizer gas flow rates were optimized to achieve the highest response. The pneumatically assisted electrospray-IMS was used for quantitative analysis of insulin, bovine serum albumin (BSA) and human serum albumin (HSA). The analysis of proteins demonstrated that sensitivity of the pneumatically assisted electrospray-IMS is two to three times higher than that of conventional electrospray ionization (ESI) coupled to IMS. The linear dynamic ranges for insulin, BSA and HSA were found to be 1-75, 5-100 and 2-100 μg mL(-1) with detection limits of 0.3, 1 and 0.6 μg mL(-1), respectively. The relative standard deviations (RSD) for five replicate measurements of insulin, BSA, and HSA at 25 μg mL(-1) were recorded as 3, 4 and 1.5%, respectively. The proposed method can be considered as an alternative way for quantitative protein analysis.
在这项工作中，引入了使用离子淌度谱（IMS）对完整蛋白质进行定量分析的方法。为此，构建了一种具有全新设计的气动辅助电喷雾电离源，并对其进行了优化以获得最高的响应。气动辅助电喷雾-IMS 被用于胰岛素、牛血清白蛋白（BSA）和人血清白蛋白（HSA）的定量分析。对蛋白质的分析表明，气动辅助电喷雾-IMS 的灵敏度比传统的与 IMS 耦合的电喷雾电离（ESI）高两到三倍。胰岛素、BSA 和 HSA 的线性动态范围分别为 1-75、5-100 和 2-100 μg mL(-1)，检测限分别为 0.3、1 和 0.6 μg mL(-1)。在 25 μg mL(-1)时，胰岛素、BSA 和 HSA 的五个重复测量的相对标准偏差（RSD）分别记录为 3%、4%和 1.5%。该方法可以被视为定量蛋白质分析的一种替代方法。
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