Department of Chemistry, Isfahan University of Technology, Isfahan 84154-83111, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Apr 15;925:26-32. doi: 10.1016/j.jchromb.2013.02.018. Epub 2013 Feb 26.
An extraction method based on aptamer sorbent followed by electrospray ionization-ion mobility spectrometry (ESI-IMS) has been developed for the analysis of tetracycline in human urine and plasma samples. The effect of extraction parameters on the extraction efficiency including washing (solvent type and volume) and elution (solvent type, volume and flow rate) were investigated. Under the optimized conditions, the linear dynamic ranges for tetracycline in urine and plasma samples were found to be 0.05-5.00 μg/mL and 0.10-5.00 μg/mL with detection limits of 0.019 and 0.037 μg/mL, respectively. The extraction efficiency was 86.5% for urine and it was 82.8% for plasma samples. The relative standard deviation was 5.9% and 6.3% for six replicate measurements of tetracycline at 1 and 2 μg/mL in urine and plasma samples, respectively.
建立了一种基于适体吸附剂的提取方法，结合电喷雾电离-离子淌度谱（ESI-IMS），用于分析人尿液和血浆样品中的四环素。考察了提取参数对提取效率的影响，包括洗涤（溶剂类型和体积）和洗脱（溶剂类型、体积和流速）。在优化条件下，四环素在尿液和血浆样品中的线性动态范围分别为 0.05-5.00 μg/mL 和 0.10-5.00 μg/mL，检测限分别为 0.019 和 0.037 μg/mL。尿液的提取效率为 86.5%，血浆样品的提取效率为 82.8%。在尿液和血浆样品中，四环素浓度为 1 和 2 μg/mL 时，重复测量 6 次的相对标准偏差分别为 5.9%和 6.3%。
