基于磁性石墨烯纳米片的电化学发光免疫分析测定癌症标志物，该方法使用 CdTe 量子点包覆的硅纳米球作为标记物。

Magnetic graphene nanosheets based electrochemiluminescence immunoassay of cancer biomarker using CdTe quantum dots coated silica nanospheres as labels.

机构信息

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

出版信息

Talanta. 2012 Sep 15;99:512-9. doi: 10.1016/j.talanta.2012.06.021. Epub 2012 Jun 26.

DOI:10.1016/j.talanta.2012.06.021

PMID:22967587

Abstract

A highly sensitive electrochemiluminescence (ECL) immunosensor for the detection of prostate specific antigen (PSA) was designed using biofunctionalized magnetic graphene nanosheets (G@Fe(3)O(4)) as immunosensing probes and CdTe quantum dots coated silica nanospheres (Si/QDs) as signal amplification labels. In this work, a sandwich-type immunosensor was fabricated, which was assembled on the surface of indium tin oxide glass (ITO). The analyte was detected in a home-made flow injection ECL (FI-ECL) cell through the immunosensor. Owing to the signal amplification of G@Fe(3)O(4) composite and Si/QDs, the ECL measurement showed a great increase in detection signals compared with the unampliﬁed method. Under optimal conditions, a wide detection range (0.003-50 ng mL(-1)) and a low detection limit (0.72 pg mL(-1)) were obtained through the sandwich-type immunosensor. The proposed strategy successfully demonstrated a reproducible, speciﬁc, and potent method that can be expanded to detect other proteins.

摘要

一种基于生物功能化磁性石墨烯纳米片（G@Fe(3)O(4)）作为免疫传感探针和碲化镉量子点包覆的硅纳米球（Si/QDs）作为信号放大标签的高灵敏度电化学发光（ECL）免疫传感器被设计用于检测前列腺特异性抗原（PSA）。在这项工作中，三明治型免疫传感器被组装在氧化铟锡（ITO）玻璃表面。通过免疫传感器，在自制的流动注射电化学发光（FI-ECL）池内检测分析物。由于 G@Fe(3)O(4) 复合纳米材料和 Si/QDs 的信号放大作用，与非放大方法相比，ECL 测量显示出了检测信号的显著增加。在最佳条件下，通过三明治型免疫传感器获得了宽的检测范围（0.003-50 ng mL(-1))和低的检测限（0.72 pg mL(-1))。所提出的策略成功地证明了一种可重复性好、特异性强、潜力巨大的方法，该方法可扩展用于检测其他蛋白质。