Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry (IUQFN-UCO) Campus of Rabanales, Marie Curie Building (Annex) University of Córdoba, E-14071 Córdoba, Spain.
Talanta. 2012 Sep 15;99:538-43. doi: 10.1016/j.talanta.2012.06.029. Epub 2012 Jun 20.
The preparation of hybrid nanostructures formed by gold nanoparticles (AuNPs) into biotinylated liposomes and their analytical application are presented. The surface of negatively charged AuNPs was modified with 1-dodecanethiol and the NPs were encapsulated into biotinylated liposomes using the rapid solvent evaporation method. Liposomes were resized by both mechanical shaking and ultrasound treatments and filled liposomes were separated from empty liposomes using sucrose density gradient centrifugation. The analytical usefulness of AuNP-liposome hybrids as amplification probes for biotin determination was checked using the competitive affinity reaction based on the avidin-biotin interaction and biotilynated phospholipids for the synthesis of the liposome hybrids. The method was automatized using a flow system and measuring the resonance light scattering signal. The dynamic range of the calibration graph was 0.001-20 μg mL(-1), (r(2)=0.9998, n=14), with a detection limit of 0.3 ng mL(-1). The precision, expressed as relative standard deviation (RSD%), was lower than 5% and the sampling frequency was 9 h(-1). The approach has been applied to the determination of biotin in food samples, with recovery values ranging between 88.2 and 105.2%.
介绍了由金纳米粒子（AuNPs）形成的杂交纳米结构的制备及其在分析中的应用。带负电荷的 AuNPs 的表面用 1-十二硫醇进行修饰，并用快速溶剂蒸发法将 NPs 包封入生物素化脂质体中。通过机械搅拌和超声处理对脂质体进行再尺寸化，并使用蔗糖密度梯度离心将填充的脂质体与空脂质体分离。使用基于亲和素-生物素相互作用的竞争亲和反应和生物素化磷脂合成脂质体杂交体，检查了 AuNP-脂质体杂交体作为生物素测定的放大探针的分析有用性。该方法使用流动系统和测量共振光散射信号进行自动化。校准曲线的动态范围为 0.001-20 μg mL(-1)，（r(2)=0.9998，n=14），检测限为 0.3 ng mL(-1)。精密度以相对标准偏差（RSD%）表示，低于 5%，采样频率为 9 h(-1)。该方法已应用于食品样品中生物素的测定，回收率在 88.2%至 105.2%之间。
