Clinical Laboratory Medicine Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Clin Biochem. 2012 Dec;45(18):1624-8. doi: 10.1016/j.clinbiochem.2012.08.004. Epub 2012 Aug 10.
This study aimed to investigate the association between polymorphisms of T-cell immunoglobulin and mucin domain molecule-3 (TIM-3) and Graves' disease (GD) in a Chinese population.
Genomic DNA was extracted from peripheral blood cells of the 182 GD patients and 150 control subjects. The TIM-3 gene polymorphic sites were genotyped. We also analyzed the relationships between the genotypes of each SNP and serum specific clinical variables. To detect whether the variants were associated with the TIM-3 expression, we further studied 40 patients by using the method of real-time quantitative reverse-transcription polymerase chain reaction (real-time RT-PCR).
The genotype and allele frequency of each polymorphic site were not significantly different between GD and control individuals. Furthermore, it also showed no relationship between the variants and TIM-3 mRNA expression.
Our results demonstrated that the polymorphisms of TIM-3 gene may not contribute to GD susceptibility in the Chinese Han population.
本研究旨在探讨中国人群中 T 细胞免疫球蛋白和粘蛋白结构域分子-3（TIM-3）基因多态性与格雷夫斯病（GD）之间的关系。
从 182 例 GD 患者和 150 例对照者的外周血细胞中提取基因组 DNA。对 TIM-3 基因多态性位点进行基因分型。我们还分析了每个 SNP 基因型与血清特定临床变量之间的关系。为了检测这些变异是否与 TIM-3 表达有关，我们进一步用实时定量逆转录聚合酶链反应（real-time RT-PCR）方法研究了 40 例患者。
GD 患者与对照组个体之间每个多态性位点的基因型和等位基因频率无显著差异。此外，这些变异与 TIM-3 mRNA 表达之间也没有关系。
我们的结果表明，TIM-3 基因的多态性可能不会导致中国汉族人群 GD 的易感性。
