兔腹膜间皮细胞的分离与鉴定

Isolation and characterization of rabbit peritoneal mesothelial cells.

作者信息

Setrakian S, Saunders B O, Nicosia S V

机构信息

Department of Pathology, University of South Florida College of Medicine, Tampa.

出版信息

Acta Cytol. 1990 Jan-Feb;34(1):92-100.

PMID:2296845

Abstract

Four enriched mesothelial cell populations distinguished by different size and density were obtained when a unit gravity sedimentation procedure was applied to pronase-dispersed rabbit peritoneal mesothelial cells. Cell integrity was confirmed by trypan blue, ultrastructural and biosynthetic analyses, as well as by explantation in tissue culture. The enriched mesothelial cells displayed the immunocytochemical, ultrastructural and growth characteristics of native mesothelial cells. This isolation and separation procedure should provide a valuable experimental tool to study the pathobiology of mesothelial cells.

摘要

当采用单位重力沉降程序对经链霉蛋白酶分散的兔腹膜间皮细胞进行处理时，可获得四个由不同大小和密度区分的富集间皮细胞群体。通过台盼蓝、超微结构和生物合成分析以及组织培养中的外植体培养，证实了细胞的完整性。富集的间皮细胞表现出天然间皮细胞的免疫细胞化学、超微结构和生长特征。这种分离和分选程序应为研究间皮细胞的病理生物学提供一个有价值的实验工具。