Saito Akatsuki, Kawamoto Yoshi, Higashino Atsunori, Yoshida Tomoyuki, Ikoma Tomoko, Suzaki Yuriko, Ami Yasushi, Shioda Tatsuo, Nakayama Emi E, Akari Hirofumi
Primate Research Institute, Kyoto University Inuyama, Japan.
Front Microbiol. 2012 Aug 30;3:314. doi: 10.3389/fmicb.2012.00314. eCollection 2012.
A recent study showed that the frequency of an antiretroviral factor TRIM5 gene-derived isoform, TRIMCyp, in cynomolgus macaques (Macaca fascicularis) varies widely according to the particular habitat examined. However, whether the findings actually reflect the prevalence of TRIMCyp in wild cynomolgus macaques is still uncertain because the previous data were obtained with captive monkeys in breeding and rearing facilities. Here, we characterized the TRIM5 gene in cynomolgus macaques captured in the wild, and found that the frequency of the TRIMCyp allele was comparable to those in captive monkeys. This suggests that the previous results with captive monkeys do indeed reflect the natural allele frequency and that breeding and rearing facilities may not affect the frequency of TRIM5 alleles. Interestingly, the prevalence of a minor haplotype of TRIMCyp in wild macaques from the Philippines was significantly lower than in captive ones, suggesting that it is advantageous for wild monkeys to possess the major haplotype of TRIMCyp. Overall, our results add to our understanding of the geographic and genetic prevalence of cynomolgus macaque TRIMCyp.
最近的一项研究表明，食蟹猴（猕猴属）中抗逆转录病毒因子TRIM5基因衍生的异构体TRIMCyp的频率，根据所检测的特定栖息地而有很大差异。然而，这些发现是否真的反映了野生食蟹猴中TRIMCyp的流行情况仍不确定，因为之前的数据是通过繁殖和饲养设施中的圈养猴子获得的。在这里，我们对在野外捕获的食蟹猴的TRIM5基因进行了特征分析，发现TRIMCyp等位基因的频率与圈养猴子中的频率相当。这表明之前对圈养猴子的研究结果确实反映了自然等位基因频率，并且繁殖和饲养设施可能不会影响TRIM5等位基因的频率。有趣的是，来自菲律宾的野生猕猴中TRIMCyp一种次要单倍型的流行率明显低于圈养猕猴，这表明野生猴子拥有TRIMCyp的主要单倍型是有利的。总体而言，我们的研究结果增进了我们对食蟹猴TRIMCyp的地理和遗传流行情况的理解。
