Maeda Hayato, Yamazaki Mao, Katagata Yohtaro
Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori 036-8561, Japan.
Exp Ther Med. 2012 Jan;3(1):49-52. doi: 10.3892/etm.2011.357. Epub 2011 Sep 27.
Essential oils diluted from certain plants have been shown to have antitumor activity against several human tumor cell lines. Kuromoji (Lindera umbellata) essential oil (KEO) has long been used in Japan as a traditional medicine. KEO and its major chemical constituent, linalool, were investigated in this study for their ability to induce apoptosis and differentiation in human leukemia HL-60 cells. HL-60 cells were treated with 5 or 50 μg/ml KEO or linalool for 24 or 48 h. Then, cell proliferation and apoptosis induction were estimated. In addition, HL-60 cells are known to differentiate into granulocyte or monocytes by a variety of compounds. Therefore, the effect of KEO or linalool on differentiation of HL-60 cells was assessed by Giemsa stain and a nitroblue tetrazolium reduction assay. Cells treated with KEO or linalool for 48 h showed significantly suppressed cell proliferation, with induced apoptosis. Moreover, KEO and linalool promoted cell differentiation. Treatment with KEO cells at the same dose as linalool showed an almost identical effect on HL-60 cells. These results suggest that KEO and linalool have efficacy as anticancer therapeutic products.
从某些植物中提取的稀释精油已被证明对多种人类肿瘤细胞系具有抗肿瘤活性。月桂（Lindera umbellata）精油（KEO）在日本长期以来一直被用作传统药物。本研究对KEO及其主要化学成分芳樟醇在诱导人白血病HL-60细胞凋亡和分化方面的能力进行了研究。用5或50μg/ml的KEO或芳樟醇处理HL-60细胞24或48小时。然后，评估细胞增殖和凋亡诱导情况。此外，已知HL-60细胞可通过多种化合物分化为粒细胞或单核细胞。因此，通过吉姆萨染色和硝基蓝四唑还原试验评估KEO或芳樟醇对HL-60细胞分化的影响。用KEO或芳樟醇处理48小时的细胞显示出细胞增殖明显受到抑制，并诱导了凋亡。此外，KEO和芳樟醇促进了细胞分化。用与芳樟醇相同剂量的KEO处理细胞对HL-60细胞显示出几乎相同的效果。这些结果表明，KEO和芳樟醇作为抗癌治疗产品具有疗效。
