Li Chong, Wang Tian, Jiang Na, Yu Pengfei, DU Yuan, Ren Rutong, Fu Fenghua
Department of Pharmacology, School of Pharmacy, Yantai University, Yantai 264005, P.R. China.
Exp Ther Med. 2012 Feb;3(2):269-272. doi: 10.3892/etm.2011.391. Epub 2011 Nov 28.
The present study was designed to evaluate the effects of steady and fluctuant inhibition of acetylcholinesterase (AChE) activity on neurotrophic factors in the hippocampus of juvenile mice. Steady inhibition of AChE activity was induced by an intramuscular injection of huperizine A (HupA) sustained-release microspheres. Fluctuant inhibition of AChE activity was induced by an intragastric administration of HupA tablets. Six days after cessation of steady AChE inhibition, there was a significant increase in the levels of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). In contrast, fluctuant AChE inhibition had no effect on BDNF and NGF levels. Additionally, neither steady nor fluctuant inhibition of AChE activity altered the choline acetyltransferase activity or spatial learning in juvenile mice. These findings indicate that steady and fluctuant methods of inhibition of AChE have different effects on the levels of BDNF and NGF in the hippocampus. In addition, the effects of AChE inhibitors may not improve learning in normal juvenile animals.
本研究旨在评估对幼年小鼠海马中神经营养因子，持续和波动抑制乙酰胆碱酯酶（AChE）活性的影响。通过肌肉注射石杉碱甲（HupA）缓释微球诱导AChE活性的持续抑制。通过胃内给予HupA片剂诱导AChE活性的波动抑制。在持续AChE抑制停止6天后，脑源性神经营养因子（BDNF）和神经生长因子（NGF）水平显著增加。相比之下，波动AChE抑制对BDNF和NGF水平没有影响。此外，AChE活性的持续或波动抑制均未改变幼年小鼠的胆碱乙酰转移酶活性或空间学习能力。这些发现表明，AChE抑制的持续和波动方法对海马中BDNF和NGF水平有不同影响。此外，AChE抑制剂的作用可能无法改善正常幼年动物的学习能力。
