Vega-Morales Tanausú, Guedes-Alonso Rayco, Sosa-Ferrera Zoraida, Santana-Rodríguez José Juan
Universidad de Las Palmas de Gran Canaria, Departamento de Química, 35017 Las Palmas de Gran Canaria, Spain.
J AOAC Int. 2012 Jul-Aug;95(4):1195-204. doi: 10.5740/jaoacint.11-219.
During the last two decades, a large number of publications have clearly shown that anthropogenic compounds that disrupt the endocrine system of wildlife species are a major cause for concern, and this concern has led to a demand for new screening methods. In this work, we have optimized and applied a new method to identify endocrine-disrupting chemicals (EDCs), such as nonylphenol, octylphenol, and their corresponding ethoxylates, 17alpha-ethynylestradiol, bisphenol-A, 17beta-estradiol, and estriol, in sewage samples. For the extraction and preconcentration of all analytes from the dissolved and particulate phases, we used SPE and ultrasonic assisted extraction, respectively. Identification and quantification were achieved by HPLC with fluorescence detection. Satisfactory LODs (between 0.5 and 7.6 ng/L in the dissolved phase and 12.3 and 21.4 ng/g in the particulate phase) and analyte recoveries (between 67 and 102%) were achieved for the target compounds. The optimized method was applied to the determination of EDCs in liquid sewage samples collected from July 2009 to July 2010 from a wastewater treatment plant in Las Palmas de G.C. (Spain). Concentrations of EDCs ranged from <10 to nearly 1300 ng/L in the dissolved phase, and from 0.1 to 7.7 microg/g in the suspended particulate matter.
在过去二十年中，大量出版物已清楚表明，扰乱野生动物物种内分泌系统的人为化合物是一个主要的关注原因，这种关注导致了对新筛选方法的需求。在这项工作中，我们优化并应用了一种新方法来鉴定污水样品中的内分泌干扰化学物质（EDC），如壬基酚、辛基酚及其相应的乙氧基化物、17α-乙炔雌二醇、双酚A、17β-雌二醇和雌三醇。对于从溶解相和颗粒相中提取和预浓缩所有分析物，我们分别使用了固相萃取（SPE）和超声辅助萃取。通过带荧光检测的高效液相色谱法进行鉴定和定量。目标化合物实现了令人满意的检测限（溶解相为0.5至7.6 ng/L，颗粒相为12.3至21.4 ng/g）和分析物回收率（67%至102%）。将优化后的方法应用于2009年7月至2010年7月从西班牙大加那利岛拉斯帕尔马斯的一家污水处理厂采集的液体污水样品中EDC的测定。溶解相中EDC的浓度范围为<10至近1300 ng/L，悬浮颗粒物中的浓度范围为0.1至7.7 μg/g。
