Laitinen L, Juusela H, Virtanen I
Department of Anatomy, University of Helsinki, Finland.
Anat Rec. 1990 Jan;226(1):10-7. doi: 10.1002/ar.1092260103.
The binding of a panel of blood group-reactive lectins to frozen sections of human kidney was studied with a special emphasis on reactivity with endothelia and basement membranes. The blood group A-reactive lectins, all specific for alpha-D-N-acetylgalactosamine (GalNAc), Helix aspersa (HAA), Helix pomatia (HPA), and Griffonia simplicifolia I-A4 (GSA-I-A4) agglutinins bound to the endothelium in specimens with blood groups A and AB. In other samples, these lectins reacted predominantly with tubular basement membranes, as well as with certain tubules. Both Dolichos biflorus (DBA) and Vicia villosa agglutinins (VVA), reported to react with blood group A1 substance, failed to reveal endothelia in most specimens, but bound differently to tubules in all blood groups. The blood group B-reactive lectins, specific for alpha-D-galactose (alpha-Gal) or GalNAc, respectively, GSA-I-B4 and Sophora japonica agglutinin (SJA), bound to the endothelia in specimens from blood group B or AB and in other specimens bound only to certain tubules. Among the blood group O-reactive lectins, specific for alpha-L-fucose (Fuc), Ulex europaeus I agglutinin (UEA-I) conjugates, but not other lectins with a similar nominal specificity, bound strongly to endothelia in specimens with blood group O. The UEA-I conjugates bound distinctly more faintly to endothelia in specimens of other blood groups. The present results indicate that lectins, binding to defined blood group determinants, react with endothelia in specimens of the respective blood group status. Furthermore, they suggest that basement membranes and some tubules in the human kidney show a distinct heterogeneity in their expression of saccharide residues, related to their blood group status.
研究了一组血型反应性凝集素与人肾冰冻切片的结合情况，特别关注其与内皮细胞和基底膜的反应性。血型A反应性凝集素，均对α-D-N-乙酰半乳糖胺（GalNAc）具有特异性，即欧洲蜗牛（HAA）、苹果蜗牛（HPA）和西非槐凝集素I-A4（GSA-I-A4），在血型为A和AB的标本中与内皮细胞结合。在其他样本中，这些凝集素主要与肾小管基底膜以及某些肾小管发生反应。据报道，双花扁豆（DBA）和绒毛野豌豆凝集素（VVA）与血型A1物质发生反应，但在大多数标本中未能显示内皮细胞，不过在所有血型中与肾小管的结合情况有所不同。血型B反应性凝集素，分别对α-D-半乳糖（α-Gal）或GalNAc具有特异性，即GSA-I-B4和槐凝集素（SJA），在血型为B或AB的标本中与内皮细胞结合，在其他标本中仅与某些肾小管结合。在血型O反应性凝集素中，对α-L-岩藻糖（Fuc）具有特异性的欧洲刺柏I凝集素（UEA-I）结合物，但其他具有类似名义特异性的凝集素则不然，在血型为O的标本中与内皮细胞强烈结合。UEA-I结合物在其他血型标本中与内皮细胞的结合明显较弱。目前的结果表明，与特定血型决定簇结合的凝集素，在相应血型状态的标本中与内皮细胞发生反应。此外，它们表明人肾中的基底膜和一些肾小管在糖残基表达上表现出明显的异质性，这与它们的血型状态有关。
