Gao Z Q, Guo X, Duan C, Ma W, Xu P, Wang W, Chen J C
Department of Orthopaedic Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, and Institute of Endemic Diseases, Faculty of Public Health, College of Meidicne, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shanxi Province, China.
J Int Med Res. 2012;40(4):1325-34. doi: 10.1177/147323001204000411.
To investigate cell morphology, aggrecan expression, and type I, II, III and X collagen expression in chondrocytes from adults with Kashin-Beck disease or osteoarthritis (OA).
Samples of knee articular cartilage were taken during surgery; cartilage samples obtained from fresh cadavers without arthritic disease were used as controls. Samples were digested with collagenase; isolated chondrocytes were cultured in monolayers. Aggrecan was detected by toluidine blue staining; collagen and aggrecan protein levels were evaluated by immuno cytochemistry and immuno fluorescence staining.
Samples were obtained from six participants per group. Aggrecan and type II collagen levels in chondrocytes from patients were significantly lower than those from controls, but levels of type I, III and X collagen were enhanced in patients compared with controls. Production of type III and X collagen was higher in chondrocytes from patients with Kashin-Beck disease than in those from OA patients.
Biochemical and morphological mechanisms underlying Kashin-Beck disease and OA include enhanced dedifferentiation and hypertrophy of chondrocytes, increased type I, III and X collagen levels, and suppressed type II collagen and aggrecan production compared with control samples.
研究大骨节病或骨关节炎（OA）成年患者软骨细胞的细胞形态、聚集蛋白聚糖表达以及Ⅰ型、Ⅱ型、Ⅲ型和X型胶原蛋白表达。
在手术过程中采集膝关节软骨样本；取自无关节炎疾病新鲜尸体的软骨样本用作对照。样本用胶原酶消化；分离的软骨细胞进行单层培养。通过甲苯胺蓝染色检测聚集蛋白聚糖；通过免疫细胞化学和免疫荧光染色评估胶原蛋白和聚集蛋白聚糖蛋白水平。
每组从6名参与者获取样本。患者软骨细胞中的聚集蛋白聚糖和Ⅱ型胶原蛋白水平显著低于对照组，但与对照组相比，患者的Ⅰ型、Ⅲ型和X型胶原蛋白水平升高。大骨节病患者软骨细胞中Ⅲ型和X型胶原蛋白的产生高于骨关节炎患者。
与对照样本相比，大骨节病和骨关节炎的生化和形态学机制包括软骨细胞去分化和肥大增强、Ⅰ型、Ⅲ型和X型胶原蛋白水平增加以及Ⅱ型胶原蛋白和聚集蛋白聚糖产生受到抑制。
