Jiang Janina, Kelly Kathleen A
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
J Vis Exp. 2012 Sep 3(67):e4391. doi: 10.3791/4391.
Mucosal surfaces, including in the gastrointestinal, urogenital, and respiratory tracts, provide portals of entry for pathogens, such as viruses and bacteria. Mucosae are also inductive sites in the host to generate immunity against pathogens, such as the Peyers patches in the intestinal tract and the nasal-associated lymphoreticular tissue in the respiratory tract. This unique feature brings mucosal immunity as a crucial player of the host defense system. Many studies have been focused on gastrointestinal and respiratory mucosal sites. However, there has been little investigation of reproductive mucosal sites. The genital tract mucosa is the primary infection site for sexually transmitted diseases (STD), including bacterial and viral infections. STDs are one of the most critical health challenges facing the world today. Centers for Disease Control and Prevention estimates that there are 19 million new infectious every year in the United States. STDs cost the U.S. health care system $17 billion every year, and cost individuals even more in immediate and life-long health consequences. In order to confront this challenge, a greater understanding of reproductive mucosal immunity is needed and isolating lymphocytes is an essential component of these studies. Here, we present a method to reproducibly isolate lymphocytes from murine female genital tracts for immunological studies that can be modified for adaption to other species. The method described below is based on one mouse.
包括胃肠道、泌尿生殖道和呼吸道在内的黏膜表面为病毒和细菌等病原体提供了进入途径。黏膜也是宿主中产生针对病原体免疫的诱导部位，例如肠道中的派尔集合淋巴结和呼吸道中的鼻相关淋巴组织。这一独特特性使黏膜免疫成为宿主防御系统的关键参与者。许多研究都集中在胃肠道和呼吸道黏膜部位。然而，对于生殖黏膜部位的研究却很少。生殖道黏膜是包括细菌和病毒感染在内的性传播疾病（STD）的主要感染部位。性传播疾病是当今世界面临的最严峻的健康挑战之一。疾病控制与预防中心估计，美国每年有1900万新发感染病例。性传播疾病每年给美国医疗保健系统造成170亿美元的损失，给个人带来的直接和终身健康后果的代价更高。为了应对这一挑战，需要更深入地了解生殖黏膜免疫，而分离淋巴细胞是这些研究的重要组成部分。在这里，我们介绍一种可重复地从小鼠雌性生殖道中分离淋巴细胞用于免疫学研究的方法，该方法可进行修改以适用于其他物种。以下所述方法以一只小鼠为基础。
