Masamura Noriya, Aoyagi Morihiro, Tsuge Nobuaki, Kamoi Takahiro, Imai Shinsuke
Somatech Center, House Foods Corporation, Takanodai, Yotsukaido, Chiba 284-0033, Japan.
Biosci Biotechnol Biochem. 2012;76(9):1799-801. doi: 10.1271/bbb.120338. Epub 2012 Sep 7.
We produced a single deuterated lachrymatory factor (propanthial S-oxide, m/z = 91) in a model reaction system comprising purified alliinase, lachrymatory factor synthase (LFS), and (E)-(+)-S-(1-propenyl)-L-cysteine sulfoxide ((E)-PRENCSO) in D(2)O. Onion LFS reacted with the degraded products of (E)-PRENCSO by alliinase, but not with those of (Z)-PRENCSO. These findings indicate that onion LFS is an (E)-1-propenylsulfenic acid isomerase.
我们在一个模型反应体系中制备了单一的氘代催泪因子（丙烷硫醛 S-氧化物，质荷比 = 91），该体系包含纯化的蒜氨酸酶、催泪因子合酶（LFS）以及重水（D₂O）中的 (E)-(+)-S-(1-丙烯基)-L-半胱氨酸亚砜（(E)-PRENCSO）。洋葱 LFS 与蒜氨酸酶催化 (E)-PRENCSO 降解产生的产物发生反应，但不与 (Z)-PRENCSO 的降解产物反应。这些发现表明洋葱 LFS 是一种 (E)-1-丙烯基亚磺酸异构酶。
