Department of Biochemistry and Molecular Biology, Second Military Medical University, PR China.
Biol Pharm Bull. 2012;35(11):1964-71. doi: 10.1248/bpb.b12-00368. Epub 2012 Aug 27.
Wentilactone B (EN-48-57) is one of the six derivatives separated from Aspergillus wentii (EN-48). Of these derivatives, Wentilactone B exerted a more significant antibacterial and cytotoxic activity in several tumor cell lines. The present study demonstrates that Wentilactone B could efficiently induce SMMC-7721 cells apoptosis, but not normal hepatic cells, as measured by an inverted microscope, 4',6-diamidino-2-phenylindole staining and flow cytometry. In addition, Wentilactone B could inhibit the metastasis of SMMC-7721 cells, which was detected by colony formation, scratch migration and a transwell assay, and could induce a series of intracellular events, including the down-regulation of CD44 and epidermal growth factor receptor proteins. In conclusion, Wentilactone B inhibited the growth of SMMC-7721 cells by triggering apoptosis and inhibiting metastasis.
恩替诺特 B（EN-48-57）是从曲霉菌（EN-48）中分离得到的 6 种衍生物之一。在这些衍生物中，恩替诺特 B 在几种肿瘤细胞系中表现出更强的抗菌和细胞毒性活性。本研究表明，恩替诺特 B 能够有效诱导 SMMC-7721 细胞凋亡，而对正常肝细胞没有影响，这可以通过倒置显微镜、4'，6-二脒基-2-苯基吲哚染色和流式细胞术来测量。此外，恩替诺特 B 能够抑制 SMMC-7721 细胞的转移，这可以通过集落形成、划痕迁移和 Transwell 测定来检测，并且能够诱导一系列细胞内事件，包括下调 CD44 和表皮生长因子受体蛋白。总之，恩替诺特 B 通过触发细胞凋亡和抑制转移来抑制 SMMC-7721 细胞的生长。
