Dipartimento di Chimica Organica e Industriale, University of Parma, Viale delle Scienze 17/A, 43124, Parma, Italy.
J Mass Spectrom. 2012 Sep;47(9):1170-6. doi: 10.1002/jms.3008.
Fumonisins are a family of food-borne mycotoxins with a wide spectrum of toxicological activities, produced by Fusarium verticillioides. Twenty-eight fumonisin analogues have been characterised so far, which can be separated into four main groups, identified as fumonisin A, B, C and P, being fumonisin B the most widely occurring in maize and corn-based food. In this work, major and minor fumonisin analogues produced by F. verticillioides have been determined by the development of a suitable tandem mass spectrometry procedure for target compound identification and quantification. The method has been applied to the determination of the major fumonisins in culture media of F. verticillioides and in mouldy maize. In addition to the main fumonisins produced by F. verticillioides, also secondary compounds such as FB4, FB5, FAs and FCs have been detected in both fungal liquid cultures and contaminated maize samples. The use of this method for quantification of major and minor fumonisins may be useful for an exhaustive evaluation of their occurrence and toxicological relevance in food; moreover, it may be applied for a better definition of the fumonisin biosynthetic pathways in different growing media as well as in maize.
伏马菌素是一类由串珠镰刀菌产生的具有广泛毒理学活性的食源性真菌毒素。迄今为止，已鉴定出 28 种伏马菌素类似物，可分为 4 个主要组，分别鉴定为伏马菌素 A、B、C 和 P，其中伏马菌素 B 是玉米和玉米制品中最常见的。在这项工作中，通过开发一种合适的串联质谱程序，用于目标化合物的鉴定和定量，确定了由串珠镰刀菌产生的主要和次要伏马菌素类似物。该方法已应用于测定串珠镰刀菌培养基和霉变玉米中的主要伏马菌素。除了由串珠镰刀菌产生的主要伏马菌素外，在真菌液体培养物和污染的玉米样品中还检测到了 FB4、FB5、FAs 和 FCs 等次级化合物。该方法用于定量主要和次要伏马菌素可能有助于全面评估其在食品中的存在和毒理学相关性；此外，它可用于更好地定义不同生长介质以及玉米中伏马菌素的生物合成途径。
