Yamazaki Natsuko, Kobayashi Akira, Yokogawa Hideaki, Ishibashi Yasuhisa, Oikawa Yosaburo, Tokoro Masaharu, Sugiyama Kazuhisa
Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Clin Ophthalmol. 2012;6:1365-8. doi: 10.2147/OPTH.S35258. Epub 2012 Aug 22.
The purpose of the current study was to investigate ex vivo laser confocal microscopic findings of cultured Acanthamoeba trophozoites obtained from Acanthamoeba keratitis patients.
Eight cultured samples of Acanthamoeba trophozoites from eight eyes of seven patients (mean age, 26.9 years; age range, 18-52 years) were used. Seven samples were from corneal scrapings of Acanthamoeba keratitis patients and one sample was from the solution in a soft contact lens case. Ex vivo laser confocal microscopy was performed to qualitatively evaluate the shape and degree of light reflection of the living Acanthamoeba trophozoites.
Ex vivo laser confocal microscopy demonstrated highly reflective, high-contrast Acanthamoeba trophozoites with no walls (mean size, 25.4 μm; range, 17.1-58.5 μm). The shapes of the trophozoites were highly pleomorphic, and some showed characteristic acanthopodia by laser confocal microscopy.
Ex vivo laser confocal microscopy was effective in demonstrating cultured Acanthamoeba trophozoites of various shapes and sizes. The observations of the current study may be helpful when similar structures are identified under in vivo conditions.
本研究旨在调查从棘阿米巴角膜炎患者获取的培养棘阿米巴滋养体的离体激光共聚焦显微镜检查结果。
使用了来自7例患者8只眼睛（平均年龄26.9岁；年龄范围18 - 52岁）的8个棘阿米巴滋养体培养样本。7个样本来自棘阿米巴角膜炎患者的角膜刮片，1个样本来自软性隐形眼镜盒内的溶液。进行离体激光共聚焦显微镜检查以定性评估活棘阿米巴滋养体的形状和光反射程度。
离体激光共聚焦显微镜显示无壁的、高反射性、高对比度的棘阿米巴滋养体（平均大小25.4μm；范围17.1 - 58.5μm）。滋养体形状高度多形性，一些在激光共聚焦显微镜下显示出特征性的棘状伪足。
离体激光共聚焦显微镜对于显示各种形状和大小的培养棘阿米巴滋养体有效。当在体内条件下识别出类似结构时，本研究的观察结果可能会有所帮助。
