Cavanagh H D, Petroll W M, Alizadeh H, He Y G, McCulley J P, Jester J V
Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas.
Ophthalmology. 1993 Oct;100(10):1444-54. doi: 10.1016/s0161-6420(93)31457-0.
The purpose of this article is to introduce the practicing ophthalmologist to the optical principles and images produced by a tandem scanning confocal microscope (recently approved by the Food and Drug Administration for general clinical use). The tandem scanning confocal microscope allows real-time viewing of structures in the living cornea at the cellular level in four dimensions (x, y, z, and time).
Nine patients (2 males, 7 females), ranging in age from 7 to 52 years, were examined. Images were recorded on super VHS videotape, digitized and processed on a computer workstation, and photographed for presentation.
Two-dimensional (x, y) 400 x 400-microns images (9-microns z-axis thickness) are presented for normal corneal structures and for the clinical conditions of herpetic keratitis, wound healing after myopic excimer ablation, Acanthamoeba infection, corneal dystrophies (granular, Reis-Buckler), contact lens abrasion, and the irido-corneal endothelial syndrome.
Clinical confocal microscopy has the unique potential of providing noninvasive assessment of corneal injury and disease at the cellular level that is not available currently from other technologies.
本文旨在向眼科执业医师介绍串联扫描共聚焦显微镜（最近已获美国食品药品监督管理局批准可用于一般临床）的光学原理及所产生的图像。串联扫描共聚焦显微镜可在细胞水平上对活体角膜结构进行实时的四维（x、y、z和时间）观察。
对9例年龄在7至52岁之间的患者（2例男性，7例女性）进行了检查。图像记录在超级VHS录像带上，进行数字化处理并在计算机工作站上加工，然后拍照以供展示。
展示了正常角膜结构以及疱疹性角膜炎、准分子激光原位角膜磨镶术后伤口愈合、棘阿米巴感染、角膜营养不良（颗粒状、Reis-Buckler型）、隐形眼镜擦伤和虹膜角膜内皮综合征等临床状况的二维（x、y）400×400微米图像（z轴厚度9微米）。
临床共聚焦显微镜具有在细胞水平上对角膜损伤和疾病进行无创评估的独特潜力，这是目前其他技术所无法实现的。
