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Diagnosis of TB from smear & culture negative sputum specimens by IS 6110 based PCR.通过基于IS 6110的聚合酶链反应从涂片和培养均为阴性的痰标本中诊断结核病。
Indian J Med Res. 2012;135(2):249-51.
2
Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco.摩洛哥临床标本中结核分枝杆菌传统分子诊断的评估
J Infect Dev Ctries. 2012 Jan 12;6(1):40-5. doi: 10.3855/jidc.1857.
3
IS6110-based global phylogeny of Mycobacterium tuberculosis.基于 IS6110 的结核分枝杆菌全球系统发育。
Infect Genet Evol. 2011 Jan;11(1):132-8. doi: 10.1016/j.meegid.2010.09.011. Epub 2010 Dec 4.
4
Development and application of multiprobe real-time PCR method targeting the hsp65 gene for differentiation of Mycobacterium species from isolates and sputum specimens.针对 HSP65 基因的多探针实时 PCR 方法的开发和应用，用于区分分离株和痰标本中的分枝杆菌种。
J Clin Microbiol. 2010 Sep;48(9):3073-80. doi: 10.1128/JCM.00939-10. Epub 2010 Jul 14.
5
Mycobacterial infection of the musculoskeletal tissues: the use of pathological specimens for identification of causative species by PCR-direct sequencing of 16S rDNA.肌肉骨骼组织中的分枝杆菌感染：通过聚合酶链反应直接测序 16S rDNA 对病理标本进行鉴定以确定病原体种属。
Jpn J Infect Dis. 2010 May;63(3):188-91.
6
Advances in the diagnosis of tuberculosis.结核病诊断的进展。
Respirology. 2010 Feb;15(2):220-40. doi: 10.1111/j.1440-1843.2009.01692.x.
7
Comparison of a DNA based PCR method with conventional methods for the detection of M. tuberculosis in Jos, Nigeria.基于DNA的聚合酶链反应（PCR）方法与传统方法在尼日利亚乔斯检测结核分枝杆菌的比较
J Infect Dev Ctries. 2009 Jul 1;3(6):470-5. doi: 10.3855/jidc.420.
8
Evaluation of a simplified IS6110 PCR for the rapid diagnosis of Mycobacterium tuberculosis in an area with high tuberculosis incidence.在结核病高发地区评估一种用于快速诊断结核分枝杆菌的简化IS6110聚合酶链反应
Pathol Biol (Paris). 2011 Jun;59(3):161-5. doi: 10.1016/j.patbio.2009.04.001. Epub 2009 May 23.
9
The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis.与传统方法相比，基于IS6110序列的聚合酶链反应在肺外结核病诊断中的效用。
Indian J Med Microbiol. 2008 Oct-Dec;26(4):352-5. doi: 10.4103/0255-0857.43575.
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基于 DNA 的 PCR 方法与传统方法在摩洛哥检测结核分枝杆菌的比较。

Comparison of a DNA Based PCR Approach with Conventional Methods for the Detection of Mycobacterium tuberculosis in Morocco.

机构信息

Institut National d'Hygiène, Rabat, Morocco.

出版信息

Mediterr J Hematol Infect Dis. 2012;4(1):e2012049. doi: 10.4084/MJHID.2012.049. Epub 2012 Aug 9.

DOI:10.4084/MJHID.2012.049
PMID:22973493
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3435128/
Abstract

BACKGROUND

Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment. In the objective of early TB diagnosis and rapid detection of Mycobacterium tuberculosis (MTB) in the clinical specimens, the utility of the Polymerase Chain Reaction (PCR) using the Insertion Sequence 6110 "IS6110" as target was compared to conventional methods.

METHODS

Out of 305 patients with different clinical manifestations: suspected, new, drug relapse, drug failure and chronic cases were enrolled in this study and tested by mycobacteriological and PCR techniques for the investigation about the tubercle bacilli.

RESULTS

The results of the in house "IS6110" PCR showed a good sensitivity (92.4%) and high specificity (98.0%), the positive and negative predictive values were 96.4 % and 95.3 % respectively.

CONCLUSION

This study showed clearly that the PCR testing using the "IS6110" in the routine analysis is a potential tool for the rapid TB diagnosis, especially for critical cases and would be of great interest to help the clinician in the misdiagnosed critical cases by the traditional radiology.

摘要

背景

在全球范围内，结核病（TB）是一个主要的公共卫生问题，快速诊断和适当的化疗成为提高结核病治疗效果的首要任务和严峻挑战。为了在临床标本中尽早诊断结核病并快速检测分枝杆菌（MTB），本研究比较了使用插入序列 6110（“IS6110”）作为靶标的聚合酶链反应（PCR）与传统方法的实用性。

方法

本研究纳入了 305 名具有不同临床表现的患者：疑似、新发、药物复发、药物失败和慢性病例，并通过分枝杆菌学和 PCR 技术对其进行了结核分枝杆菌检测。

结果

实验室自制的“IS6110”PCR 显示出良好的灵敏度（92.4%）和高度的特异性（98.0%），阳性预测值和阴性预测值分别为 96.4%和 95.3%。

结论

本研究清楚地表明，常规分析中使用“IS6110”的 PCR 检测是一种快速诊断结核病的潜在工具，特别是对于危急情况，对于帮助临床医生诊断传统影像学误诊的危急情况具有重要意义。