Ashby C R, Carr L A, Cook C L, Steptoe M M, Franks D D
Department of Pharmacology and Toxicology, University of Louisville, Kentucky.
J Neural Transm Gen Sect. 1990;79(1-2):41-50. doi: 10.1007/BF01250999.
The effects of plasma and an aqueous plasma fraction from patients with premenstrual syndrome (PMS) and control subjects on the uptake of 5-hydroxytryptamine (5-HT) in washed human platelets and rat forebrain synaptosomes were studied. Pre- and postmenstrual samples of unextracted plasma from the control group significantly enhanced platelet uptake of 5-HT. In contrast, an aqueous fraction following extraction of the plasma with organic solvents caused a dose-dependent decrease of 5-HT uptake. Plasma obtained from patients with PMS caused less stimulation of 5-HT uptake compared to plasma from the control group. The aqueous fraction of premenstrual plasma from patients tended to inhibit 5-HT uptake to a greater extent than a similar plasma fraction from controls. The inhibition of 5-HT uptake was associated with an increase in Km. Aqueous plasma fractions from both groups also inhibited 5-HT uptake in brain synaptosomes. However, there were no significant differences between groups. The results of the platelet study suggest that there may be quantitative differences in the plasma concentration of endogenous factors that affect 5-HT uptake between patients with PMS and control subjects and that such differences may explain the previously reported alteration of platelet 5-HT uptake and content associated with PMS symptoms.
研究了经前期综合征（PMS）患者和对照受试者的血浆及血浆水相组分对洗涤过的人血小板和大鼠前脑突触体中5-羟色胺（5-HT）摄取的影响。对照组未提取血浆的经前和经后样本显著增强了血小板对5-HT的摄取。相比之下，用有机溶剂提取血浆后的水相组分导致5-HT摄取呈剂量依赖性降低。与对照组血浆相比，PMS患者的血浆对5-HT摄取的刺激作用较小。PMS患者经前血浆的水相组分比对照组类似血浆组分更倾向于更大程度地抑制5-HT摄取。5-HT摄取的抑制与Km的增加有关。两组的血浆水相组分也抑制了脑突触体中5-HT的摄取。然而，组间无显著差异。血小板研究结果表明，PMS患者和对照受试者之间影响5-HT摄取的内源性因子血浆浓度可能存在定量差异，且这种差异可能解释了先前报道的与PMS症状相关的血小板5-HT摄取和含量的改变。
