Ye Xiyun, Wang Luyang, Dang Yongyan, Liu Bei, Zhao Di
School of Life Science, East China Normal University, Shanghai, China.
Photomed Laser Surg. 2012 Oct;30(10):604-9. doi: 10.1089/pho.2012.3221. Epub 2012 Sep 13.
The objective of this study was to evaluate the changes of collagen expression and its possible molecular mechanism in the rat skin induced by 1064 nm Q-switched Nd:YAG laser treatments.
The dorsal skin of Sprague-Dawley (SD) rats was irradiated with the 1064 nm laser at fluences of 0, 0.6, 1.5, and 2.5 J/cm2, respectively. Then biochemical analysis was used to quantify hydroxyproline content in the skin. The mRNA expressions of procollagen, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs) were analyzed by using reverse transcription-polymerase chain reaction (RT-PCR). The activities of mitogen-activated protein kinase (MAPK) family members were detected by Western blot analysis.
The 1064 nm laser treatments led to a marked increase in collagen content in a dose-dependent manner. The expression of types I and III collagen, TIMP1 and TIMP2, in the skin was markedly upregulated, whereas the expression of MMP2 and MMP3 was significantly decreased after laser treatments. Both extracellular signal-related kinase (Erk)1/2 and JNK MAPK pathways were activated by the 1064 nm laser irradiation.
The 1064 nm laser irradiation could markedly increase collagen synthesis and inhibit collagen degradation. The activation of Erk1/2 and JNK MAPK seems to play a role in collagen production in the rat skin, induced by the 1064 nm laser.
本研究旨在评估1064nm调Q Nd:YAG激光治疗对大鼠皮肤中胶原蛋白表达的变化及其可能的分子机制。
分别以0、0.6、1.5和2.5J/cm²的能量密度用1064nm激光照射斯普拉格-道利（SD）大鼠的背部皮肤。然后采用生化分析法定量皮肤中的羟脯氨酸含量。使用逆转录-聚合酶链反应（RT-PCR）分析前胶原蛋白、基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）的mRNA表达。通过蛋白质印迹分析检测丝裂原活化蛋白激酶（MAPK）家族成员的活性。
1064nm激光治疗导致胶原蛋白含量呈剂量依赖性显著增加。激光治疗后，皮肤中I型和III型胶原蛋白、TIMP1和TIMP2的表达明显上调，而MMP2和MMP3的表达显著降低。1064nm激光照射激活了细胞外信号调节激酶（Erk）1/2和JNK MAPK途径。
1064nm激光照射可显著增加胶原蛋白合成并抑制胶原蛋白降解。Erk1/2和JNK MAPK的激活似乎在1064nm激光诱导的大鼠皮肤胶原蛋白生成中起作用。
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