Department of Women's and Children's Health, Division of Pediatric Surgery, Uppsala University, SE-751 85 Uppsala, Sweden.
J Pediatr Surg. 2012 Sep;47(9):1646-51. doi: 10.1016/j.jpedsurg.2012.03.086.
BACKGROUND/PURPOSE: Necrotizing enterocolitis (NEC) represents one of the gravest complications in premature infants and carries significant morbidity and mortality. There is a great need for improved diagnostic methods to reduce the severity and incidence of NEC. The aim of the study was to investigate if intraluminal microdialysis can detect intestinal ischemia in newborn rats with induced experimental NEC.
The studies were performed on 1-day-old Sprague-Dawley rat pups. Experimental NEC was induced using hypoxia/reoxygenation treatment. Microdialysis catheters were rectally inserted and placed in the rectosigmoid part of the colon. Microdialysate levels of glucose, lactate, pyruvate, and glycerol were measured. Intestinal specimens were collected at the end of the experiments for microscopic evaluation.
Intraluminal microdialysis revealed signs of intestinal hypoxia and cellular damage, with a marked increase of lactate and glycerol. Microscopic evaluation confirmed intestinal damage in the NEC group.
Intraluminal microdialysis can detect intestinal hypoxic stress and mucosal cell membrane decay in a rat model of NEC. Intestinal intraluminal microdialysis is easily accessible through the rectum and may be a useful noninvasive complement to other methods in the assessment of NEC.
背景/目的：坏死性小肠结肠炎（NEC）是早产儿最严重的并发症之一，具有较高的发病率和死亡率。因此，急需开发更有效的诊断方法来降低 NEC 的严重程度和发病率。本研究旨在探讨腔内微透析是否可以检测到实验性 NEC 新生大鼠的肠道缺血。
该研究使用 1 日龄的 Sprague-Dawley 大鼠幼仔进行。使用缺氧/复氧处理诱导实验性 NEC。将微透析导管经直肠插入并放置在直肠乙状结肠部分。测量微透析液中葡萄糖、乳酸、丙酮酸和甘油的水平。实验结束时收集肠道标本进行显微镜评估。
腔内微透析显示出肠道缺氧和细胞损伤的迹象，乳酸和甘油明显增加。显微镜评估证实了 NEC 组的肠道损伤。
腔内微透析可以检测到 NEC 大鼠模型中的肠道缺氧应激和黏膜细胞膜衰竭。肠道腔内微透析通过直肠很容易进行，可能是评估 NEC 的其他方法的有用非侵入性补充手段。
