生后睾丸小管中基质金属蛋白酶是否对生殖细胞成熟是必需的？
Is matrix metalloproteinase required in postnatal testicular tubules for germ cell maturation?
机构信息
F. Douglas Stephens Surgical Research Laboratory, Murdoch Childrens Research Institute, Melbourne 3052, Australia.
出版信息
J Pediatr Surg. 2012 Sep;47(9):1724-9. doi: 10.1016/j.jpedsurg.2012.03.062.
BACKGROUND/AIM: Cryptorchidism may cause infertility by failed transformation of neonatal gonocytes into adult dark spermatogonia, the putative stem cells for spermatogenesis. Gonocytes migrate centrifugally to the tubular basement membrane to become adult dark spermatogonia. Regulation of this transformation remains unknown. We aimed to investigate neonatal rodent testis matrix metalloproteinase (MMP) production to see whether MMPs loosen extracellular matrix between Sertoli cells to facilitate gonocyte movement.
METHODS
Sprague-Dawley rat testes (n = 4-6 per group) were collected at embryonic day 19 (E19) and postnatal (P) days P0 to 10 for immunohistochemistry. Immunofluorescent confocal images were captured for presence of membrane type 1 MMP (MT1-MMP), matrix metalloproteinase 2 (MMP2), tissue inhibitor of metalloproteinase 2 (TIMP2), mouse VASA homologue, anti-Müllerian hormone, and androgen receptor in tissue sections. Testicular proteins were analyzed by immunoblotting.
RESULTS
Membrane type 1 MMP was strongly present in gonocytes at E19 then decreased, whereas it increased in testicular somatic cells from P0 to P10. Testicular protein levels of MT1-MMP, MMP2, and androgen receptor were constant from E19 to P10. Anti-Müllerian hormone protein sharply decreased after P2, whereas TIMP2 gradually increased from E19 to P10. Gonocytes migrated to basement membrane at P2 to P6.
CONCLUSION
Membrane type 1 MMP, MMP2, and TIMP2 were present in testis from E19 to P10 during gonocyte migration and transformation into spermatogenic stem cells. Increased knowledge about germ cell development may aid efforts to improve fertility in cryptorchidism.
背景/目的：隐睾症可能导致新生儿生殖细胞向成年暗精原细胞（精原干细胞）的转化失败，从而导致不育。生殖细胞向心性迁移至管基底膜成为成年暗精原细胞。这种转化的调节机制尚不清楚。我们旨在研究新生啮齿动物睾丸基质金属蛋白酶（MMP）的产生情况，以观察 MMP 是否可以松解生精细胞之间的细胞外基质，从而促进生殖细胞的迁移。
方法
收集 Sprague-Dawley 大鼠胚胎 19 日龄（E19）和出生后（P）天 P0 至 10 天的睾丸组织（每组 4-6 只），进行免疫组织化学染色。捕获免疫荧光共聚焦图像，以检测组织切片中膜型 1 基质金属蛋白酶（MT1-MMP）、基质金属蛋白酶 2（MMP2）、金属蛋白酶组织抑制剂 2（TIMP2）、小鼠 VASA 同源物、抗苗勒管激素和雄激素受体的存在。通过免疫印迹分析睾丸蛋白。
结果
E19 时，生殖细胞中存在强烈的 MT1-MMP，随后减少，而从 P0 至 P10 时睾丸体细胞中的 MT1-MMP 增加。E19 至 P10 时，MT1-MMP、MMP2 和雄激素受体的睾丸蛋白水平保持不变。抗苗勒管激素蛋白在 P2 后急剧下降，而 TIMP2 从 E19 至 P10 逐渐增加。生殖细胞在 P2 至 P6 时迁移到基底膜。
结论
在生殖细胞迁移和向精原干细胞转化过程中，E19 至 P10 时睾丸中存在 MT1-MMP、MMP2 和 TIMP2。增加对生殖细胞发育的认识可能有助于提高隐睾症的生育能力。
Zotero 插件