非哺乳动物胃饥饿素的测定
Determination of nonmammalian ghrelin.
作者信息
Kaiya Hiroyuki, Hosoda Hiroshi, Kangawa Kenji, Miyazato Mikiya
机构信息
Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan.
出版信息
Methods Enzymol. 2012;514:75-87. doi: 10.1016/B978-0-12-381272-8.00005-2.
Ghrelin is a peptide with a unique molecular modification by a fatty acid such as n-octanoic acid. The acyl modification is necessary for ghrelin to bind to its receptor (growth hormone secretagogue-receptor 1a, GHS-R1a) and to induce subsequent intracellular Ca(2+) signaling. Ghrelin is widely expressed in mammals as well as in nonmammalian vertebrates. In our laboratory, a method for efficient purification of ghrelin from a small amount of tissues has been established. Here, we introduce the identification process of ghrelin in nonmammalian vertebrates.
胃饥饿素是一种经脂肪酸（如正辛酸）进行独特分子修饰的肽。这种酰基修饰对于胃饥饿素与其受体（生长激素促分泌素受体1a，GHS-R1a）结合以及诱导随后的细胞内Ca(2+)信号传导是必需的。胃饥饿素在哺乳动物以及非哺乳动物脊椎动物中广泛表达。在我们实验室，已经建立了一种从少量组织中高效纯化胃饥饿素的方法。在此，我们介绍非哺乳动物脊椎动物中胃饥饿素的鉴定过程。
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