Tomiyama Y, Take H, Ikeda H, Mitani T, Furubayashi T, Mizutani H, Yamamoto N, Tandon N N, Sekiguchi S, Jamieson G A
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Blood. 1990 Feb 1;75(3):684-7.
We describe the membrane localization of a new platelet-specific alloantigen, designated Naka, that is involved in refractoriness to HLA-matched platelet transfusions. By indirect immunoprecipitation, anti-Naka antibody precipitated a single, radiolabeled platelet membrane protein with a molecular weight (mol wt) of 91 Kd from Naka-positive platelets. When radiolabeled Naka-negative platelets were used as a source of target antigens, no radiolabeled proteins were precipitated. The analyses using nonreduced-reduced two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and using rabbit antiglycoprotein (GP)IV demonstrated that this protein corresponds to GPIV (alternatively GPIIIb). Furthermore, in dot immunobinding, anti-Naka antibody bound to purified GPIV. Our results provide definitive evidence that the Naka alloantigen is carried on GPIV. These results also demonstrate that, on occasion, antibodies against GPIV may play an important role in refractoriness to platelet transfusions.
我们描述了一种新的血小板特异性同种抗原（命名为中田抗原）的膜定位，该抗原与对 HLA 匹配的血小板输注产生不应性有关。通过间接免疫沉淀法，抗中田抗体从阳性血小板中沉淀出一种单一的、放射性标记的分子量为 91 Kd 的血小板膜蛋白。当使用放射性标记的中田阴性血小板作为靶抗原来源时，未沉淀出放射性标记蛋白。使用非还原-还原二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）以及兔抗糖蛋白（GP）IV 进行的分析表明，该蛋白对应于 GPIV（也称为 GPIIIb）。此外，在斑点免疫结合实验中，抗中田抗体与纯化的 GPIV 结合。我们的结果提供了确凿证据，表明中田同种抗原存在于 GPIV 上。这些结果还表明，针对 GPIV 的抗体有时可能在血小板输注不应性中起重要作用。
