Lakatos Andrea, Mörs Karsten, Glaubitz Clemens
Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.
Methods Mol Biol. 2012;914:65-86. doi: 10.1007/978-1-62703-023-6_5.
Solid-state NMR is an established method for biophysical studies of membrane proteins within the lipid bilayers and an emerging technique for structural biology in general. In particular magic angle sample spinning has been found to be very useful for the investigation of large membrane proteins and their interaction with small molecules within the lipid bilayer. Using a number of examples, we illustrate and discuss in this chapter, which information can be gained and which experimental parameters need to be considered when planning such experiments. We focus especially on the interaction of diffusive ligands with membrane proteins.
固态核磁共振是研究脂质双层中膜蛋白生物物理特性的既定方法，总体而言也是结构生物学领域一种新兴的技术。特别是发现魔角样品旋转对于研究大型膜蛋白及其在脂质双层中与小分子的相互作用非常有用。在本章中，我们通过若干实例来说明并讨论在规划此类实验时可以获得哪些信息以及需要考虑哪些实验参数。我们特别关注扩散性配体与膜蛋白的相互作用。
