Lee Hyun-Jin, Park Chul-Hong, Son Hyung-U, Heo Jin-Chul, Nam Sung-Hee, Lee Kwang-Gil, Yeo Joo-Hong, Yoon Cheol-Sik, Kim Jong-Myeung, Shin Yong-Kyu, Kim Si-Oh, Lee Sang-Han
Department of Food Science and Biotechnology;
Exp Ther Med. 2011 Mar;2(2):357-362. doi: 10.3892/etm.2011.214. Epub 2011 Jan 20.
The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.
本研究的目的是调查粉拟青霉J3培养滤液的抗肿瘤作用。使用B16黑色素瘤细胞进行了各种抗肿瘤试验。粉拟青霉J3显著降低了伤口愈合能力、侵袭性和血管生成活性，这在伤口愈合、人脐静脉内皮细胞和侵袭试验中得到了证实。粉拟青霉J3以浓度依赖的方式强烈抑制细胞迁移、管形成和血管生成过程。酶谱分析还表明基质金属蛋白酶-9（MMP-9，一种92 kDa的明胶酶）的表达降低。综上所述，结果表明粉拟青霉J3的抗肿瘤活性源于B16F10细胞中MMP-9表达的降低。
