吞噬与细胞运动因子1的过表达促进肝细胞癌的细胞侵袭和迁移。
Overexpression of engulfment and cell motility 1 promotes cell invasion and migration of hepatocellular carcinoma.
作者信息
Jiang Jiarui, Liu Guoqing, Miao Xiongying, Hua Songwen, Zhong Dewu
机构信息
Department of Hepatobiliary Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
出版信息
Exp Ther Med. 2011 May;2(3):505-511. doi: 10.3892/etm.2011.229. Epub 2011 Mar 18.
Abstract
Engulfment and cell motility 1 (Elmo1) has been linked to the invasive phenotype of glioma cells. The use of Elmo1 inhibitors is currently being evaluated in hepato-cellular carcinoma (HCC), but the molecular mechanisms of their therapeutic effect have yet to be determined. Elmo1 expression in HCC tissue samples from 131 cases and in 5 HCC cell lines was determined by immunohistochemistry, quantitative RT-PCR and Western blotting. To functionally characterize Elmo1 in HCC, Elmo1 expression in the HCCLM3 cell line was blocked by siRNA. Cell migration was measured by wound healing and transwell migration assays in vitro. Elmo1 overexpression was significantly correlated with cell invasion and the poor prognosis of HCC. Elmo1-siRNA-treated HCCLM3 cells demonstrated a reduction in cell migration. The present study demonstrated for the first time that the suppression of Elmo1 expression inhibits cell invasion in HCC.
摘要
吞噬与细胞运动蛋白1（Elmo1）与胶质瘤细胞的侵袭表型有关。目前正在肝细胞癌（HCC）中评估Elmo1抑制剂的使用，但它们治疗效果的分子机制尚未确定。通过免疫组织化学、定量逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法测定了131例HCC组织样本和5种HCC细胞系中Elmo1的表达。为了在功能上表征HCC中的Elmo1，通过小干扰RNA（siRNA）阻断了HCCLM3细胞系中Elmo1的表达。通过体外伤口愈合和Transwell迁移试验测量细胞迁移。Elmo1过表达与HCC细胞侵袭及预后不良显著相关。经Elmo1-siRNA处理的HCCLM3细胞表现出细胞迁移减少。本研究首次证明，抑制Elmo1表达可抑制HCC细胞的侵袭。
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RhoGTPases and Rho-effectors in hepatocellular carcinoma metastasis: ROCK N'Rho move it.RhoGTPases 和 Rho 效应物在肝细胞癌转移中的作用：ROCK N'Rho 推动其转移。
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