鱼精蛋白和受精素mRNA：辅助生殖技术结果的潜在生物标志物。

Protamine and fertilin mRNA: potential biomarkers of assisted reproductive technology outcomes.

作者信息

Sukhikh G T, Bourmenskaya O V, Smolnikova V Yu, Krasnoschoka O E, Trofimov D Yu, Donnikov A E, Kalinina E A

机构信息

V. I. Kulakov Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health and Social Development of the Russian Federation, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2012 Aug;153(4):513-5. doi: 10.1007/s10517-012-1754-0.

DOI:10.1007/s10517-012-1754-0

PMID:22977858

Abstract

We studied the relationship between the levels of protamines 1 and 2 (PRM1 and PRM2) and fertilin-β (ADAM-2) mRNA expression and outcomes of infertility treatment using assisted reproductive technologies was studied. Analysis of the relationships between the outcomes of in vitro fertilization and embryo transfer and profiles of the expression of seminal genes PRM1, PRM2, ADAM-2 mRNA, evaluated by reverse transcription quantitative PCR was carried out in 79 couples. Significant differences in the expression of seminal PRM1, PRM2, ADAM-2 mRNA were detected in couples with different outcomes of in vitro fertilization and embryo transfer. The levels of seminal gene expression are potential predictors of the efficiency of in vitro fertilization and embryo transfer.

摘要

我们研究了鱼精蛋白1和2（PRM1和PRM2）水平与受精素-β（ADAM-2）mRNA表达之间的关系，并研究了使用辅助生殖技术的不孕症治疗结果。通过逆转录定量PCR评估，对79对夫妇进行了体外受精和胚胎移植结果与精液基因PRM1、PRM2、ADAM-2 mRNA表达谱之间关系的分析。在体外受精和胚胎移植结果不同的夫妇中，检测到精液PRM1、PRM2、ADAM-2 mRNA表达存在显著差异。精液基因表达水平是体外受精和胚胎移植效率的潜在预测指标。