Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-araba, Nigeria.
Pharm Biol. 2013 Jan;51(1):49-57. doi: 10.3109/13880209.2012.704518. Epub 2012 Sep 14.
Roots of Combretum mucronatum Schumach. & Thonn. (Combretaceae) and Capparis thonningii Schum. (Capparaceae) are used in southwest Nigeria in the treatment of inflammatory disorders and mental illness.
This study evaluated the antidementic effect of the methanol root extracts of C. mucronatum and C. thonningii on scopolamine (3 mg/kg, i.p.) induced memory impairment in mice.
The effect of C. mucronatum and C. thonningii (50-200 mg/kg) administered orally for 3 days on memory impairments induced in mice by scopolamine was assessed in the passive avoidance and Morris water maze test and compared with that of tacrine (5 mg/kg, i.p.). The activities of acetylcholinesterase (AchE) and antioxidant enzymes were estimated in the brain after the completion of behavioral studies.
C. mucronatum and C. thonningii root extracts (50-200 mg/kg) reversed scopolamine-induced memory deficit with significant (p < 0.05) increase in transfer latency in passive avoidance test. Similarly, the extracts (200 mg/kg) ameliorated memory deficit as a result of significant (p < 0.001) decrease in escape latency and path length in Morris water maze test. The increased AChE activity induced by scopolamine was significantly (p < 0.05) inhibited by C. mucronatum and C. thonningii (100 and 200 mg/kg) treatment which was similar to the effect of tacrine. Both extracts significantly (p < 0.05) attenuated scopolamine-induced increase in oxidative stress parameters as well as restoration of glutathione activity.
C. mucronatum and C. thonningii extracts possess significant anticholinesterase, antioxidant and antidementic properties, which may be useful in the management of Alzheimer's disease.
在尼日利亚西南部，Combretum mucronatum Schumach. & Thonn.（使君子科）和 Capparis thonningii Schum.（山柑科）的根被用于治疗炎症性疾病和精神疾病。
本研究评估了甲醇根提取物对 Combretum mucronatum 和 Capparis thonningii 对东莨菪碱（3mg/kg，腹腔注射）诱导的小鼠记忆障碍的抗痴呆作用。
通过被动回避和 Morris 水迷宫试验评估 C. mucronatum 和 C. thonningii（50-200mg/kg）口服给药 3 天对东莨菪碱诱导的小鼠记忆障碍的影响，并与他克林（5mg/kg，腹腔注射）进行比较。在行为研究完成后，估计大脑中的乙酰胆碱酯酶（AchE）和抗氧化酶的活性。
C. mucronatum 和 C. thonningii 根提取物（50-200mg/kg）逆转了东莨菪碱诱导的记忆缺陷，使被动回避试验中的转移潜伏期显著增加（p<0.05）。同样，提取物（200mg/kg）改善了由于东莨菪碱引起的记忆缺陷，表现为逃避潜伏期和 Morris 水迷宫试验中的路径长度显著减少（p<0.001）。东莨菪碱诱导的 AchE 活性增加被 C. mucronatum 和 C. thonningii（100 和 200mg/kg）治疗显著抑制（p<0.05），这与他克林的作用相似。两种提取物均显著降低东莨菪碱诱导的氧化应激参数的增加以及谷胱甘肽活性的恢复（p<0.05）。
C. mucronatum 和 C. thonningii 提取物具有显著的抗胆碱酯酶、抗氧化和抗痴呆特性，可用于治疗阿尔茨海默病。
