Olatunji Lawrence A, Usman Taofeek O, Adebayo Joseph O, Olatunji Victoria A
Department of Physiology, University of Ilorin, Ilorin, Nigeria.
Zhong Xi Yi Jie He Xue Bao. 2012 Sep;10(9):1049-55.
To investigate the effects of oral administration of aqueous extract of Hibiscus sabdariffa on renal Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase activities in rats.
The 25 and 50 mg/(kg·d) of aqueous extracts of H. sabdariffa were respectively given to rats in the experimental groups for 28 d, and rats in the control group received an appropriate volume of distilled water as vehicle. Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase activities in the kidney were assayed by spectrophotometric method.
Administrations of 25 and 50 mg/(kg·d) of aqueous extract of H. sabdariffa significantly decreased the Ca(2+)-Mg(2+)-ATPase activity in the kidney of rats (P<0.05). However, the renal Na(+)-K(+)-ATPase activity of the experimental rats was not affected by either dose of the extract. And the plasma Na(+), K(+) and Ca(2+) levels of the experimental rats had no significant changes. Administration of either dose of the extract did not result in any significant changes in body and kidney weights, the concentrations of plasma albumin and total protein, and alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase activities. However, concentrations of creatinine and urea were significantly reduced by 50 mg/kg of the extract (P<0.05).
The present study indicates that oral administration of aqueous extract of H. sabdariffa may preserve the renal function despite a decreased renal Ca(2+)-Mg(2+)-ATPase activity.
研究口服玫瑰茄水提取物对大鼠肾脏钠钾ATP酶和钙镁ATP酶活性的影响。
将实验组大鼠分别给予25和50毫克/（千克·天）的玫瑰茄水提取物，连续给药28天，对照组大鼠给予适量蒸馏水作为溶剂。采用分光光度法测定肾脏中钠钾ATP酶和钙镁ATP酶的活性。
给予25和50毫克/（千克·天）的玫瑰茄水提取物显著降低了大鼠肾脏中钙镁ATP酶的活性（P<0.05）。然而，提取物的任何剂量均未影响实验大鼠肾脏的钠钾ATP酶活性。并且实验大鼠的血浆钠、钾和钙水平没有显著变化。给予任何剂量的提取物均未导致大鼠体重、肾脏重量、血浆白蛋白和总蛋白浓度以及碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶活性发生任何显著变化。然而，50毫克/千克的提取物显著降低了肌酐和尿素的浓度（P<0.05）。
本研究表明，尽管肾脏钙镁ATP酶活性降低，但口服玫瑰茄水提取物可能有助于维持肾脏功能。
