Novoa E, Rodrigo R
Departamento de Medicina Experimental, Facultad de Medicina, Universidad de Chile, Santiago.
Acta Physiol Pharmacol Latinoam. 1989;39(1):15-26.
The effect of long-term ethanol feeding on the activity of (Na + K)-ATPase in cortex and outer medulla and fractional excretion of electrolytes in remnant kidney of adult rats after unilateral nephrectomy were studied. Wistar adult rats were fed 20% (v/v) aqueous ethanol solution as sole drinking fluid for 8-10 weeks. Right kidney was removed under ether anaesthesia. The animals were subjected to an acute NaCl loading by means of a continuous infusion given 2, 7 and 14 days after nephrectomy. Renal handling of electrolytes was estimated from fractional excretion of sodium and potassium. After the infusion the animals were killed and (Na + K)-ATPase and Mg2+-ATPase activities were measured in the cortex and outer medulla of the remnant kidney. Two days after nephrectomy both groups showed a gradual increase of renal (Na + K)-ATPase activity reaching 60 percent at day 14. Mg2+-ATPase activity did not change with respect to basal values. Compared to basal values the fractional excretion of sodium after nephrectomy, dropped in both groups but more significantly in the ethanol-fed rats than in the control group. Fractional excretion of potassium did not change in the control group after nephrectomy while the ethanol-fed group displayed a significative decrease at days 7 and 14. According to our results the rise in renal (Na + K)-ATPase activity is consistent with the renal sodium retention found in ethanol-fed rats.
研究了长期给予乙醇对成年大鼠单侧肾切除术后残肾皮质和外髓质中（钠+钾）-ATP酶活性以及电解质排泄分数的影响。将Wistar成年大鼠以20%（v/v）的乙醇水溶液作为唯一饮用水喂养8-10周。在乙醚麻醉下切除右肾。在肾切除术后第2、7和14天，通过持续输注对动物进行急性氯化钠负荷。根据钠和钾的排泄分数评估肾脏对电解质的处理。输注后处死动物，测定残肾皮质和外髓质中（钠+钾）-ATP酶和镁离子-ATP酶的活性。肾切除术后两天，两组的肾脏（钠+钾）-ATP酶活性均逐渐升高，在第14天达到60%。镁离子-ATP酶活性相对于基础值没有变化。与基础值相比，肾切除术后两组的钠排泄分数均下降，但乙醇喂养组比对照组下降更显著。肾切除术后对照组的钾排泄分数没有变化，而乙醇喂养组在第7天和第14天出现显著下降。根据我们的结果，肾脏（钠+钾）-ATP酶活性的升高与乙醇喂养大鼠中发现的肾脏钠潴留一致。
