Department of Anatomy and Cell Biology, Research Institute of Pharmaceutical Science, Faculty of Pharmacy, Musashino University, Nishitokyo, Tokyo, Japan.
FEBS Lett. 2012 Sep 21;586(19):3464-70. doi: 10.1016/j.febslet.2012.07.074. Epub 2012 Aug 9.
Chromatin loops formed between distant regulatory elements and promoters modulate gene expression. We identified a novel distant regulatory element located approximately 120kb downstream of the gls promoter, and examined its regulatory relevance to gls gene expression in C2C12 cells by a chromosome conformation capture assay. The distant element physically interacted with the gls promoter in myoblasts but not in myotubes. Semiquantitative analysis by real-time PCR showed more abundant gls transcripts in myoblasts than in myotubes. These findings suggest that this distant element differentially regulates gls gene expression through dynamic formation and abrogation of a chromatin loop during myogenesis.
染色质环在远距离调控元件和启动子之间形成，调节基因表达。我们鉴定了一个位于 gls 启动子下游约 120kb 的新的远距离调控元件，并通过染色体构象捕获试验检测其在 C2C12 细胞中对 gls 基因表达的调控相关性。在成肌细胞中，这个远距离元件与 gls 启动子发生物理相互作用，但在肌管中没有。实时 PCR 的半定量分析显示，成肌细胞中 gls 转录本的丰度高于肌管。这些发现表明，这个远距离元件通过在肌发生过程中动态形成和消除染色质环，差异调节 gls 基因表达。
