Department of Bioenvironmental Chemistry and Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 561-756, South Korea.
J Food Prot. 2012 Jul;75(7):1258-62. doi: 10.4315/0362-028X.JFP-12-040.
The acaricidal activities of materials derived from Pyrus ussuriensis fruits were evaluated against Tyrophagus putrescentiae and compared with that of commercial acaricide (benzyl benzoate). On the basis of the 50 % lethal dose (LD(50)) values, the ethyl acetate fraction of the fractions obtained from an aqueous extract of P. ussuriensis fruits had the highest acaricidal activity (16.32 μg/cm(2)) against T. putrescentiae. The acaricidal constituent of P. ussuriensis fruits was isolated by chromatographic techniques and identified as 1,4-benzoquinone. On the basis of the LD(50) values, 1,4-benzoquinone (1.98 μg/cm(2)) was 5.9 times more toxic than benzyl benzoate (11.69 μg/cm(2)), followed by 2-isopropyl-5-methyl-1,4-benzoquinone (3.29 μg/cm(2)), and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (5.03 μg/cm(2)) against T. putrescentiae in the fumigant bioassay. In a filter paper bioassay, the acaricidal activity of 1,4-benzoquinone (0.07 μg/cm(2)) was 120.1 times more effective than that of benzyl benzoate (8.41 μg/cm(2)), followed by 2-isopropyl-5-methyl-1,4-benzoquinone (0.11 μg/cm(2)) and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (0.30 μg/cm(2)) against T. putrescentiae. These results demonstrate that P. ussuriensis fruit-derived material and its derivatives have potential as new preventive agents for the control of stored food mites.
评估了源自梨果的材料对腐食酪螨的杀螨活性，并与商业杀螨剂（苯甲酸苄酯）进行了比较。根据 50%致死剂量（LD50）值，梨果水提物的乙酸乙酯馏分对腐食酪螨具有最高的杀螨活性（16.32μg/cm2）。通过色谱技术分离梨果的杀螨成分，鉴定为 1,4-苯醌。根据 LD50 值，1,4-苯醌（1.98μg/cm2）比苯甲酸苄酯（11.69μg/cm2）毒 5.9 倍，其次是 2-异丙基-5-甲基-1,4-苯醌（3.29μg/cm2）和 2,3-二甲氧基-5-甲基-1,4-苯醌（5.03μg/cm2）。在熏气生物测定中，1,4-苯醌（0.07μg/cm2）的杀螨活性比苯甲酸苄酯（8.41μg/cm2）高 120.1 倍，其次是 2-异丙基-5-甲基-1,4-苯醌（0.11μg/cm2）和 2,3-二甲氧基-5-甲基-1,4-苯醌（0.30μg/cm2）。这些结果表明，梨果来源的材料及其衍生物具有作为控制储存食品螨类的新型预防剂的潜力。
