Instituto Agronômico de Campinas, Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Caixa Postal 28, 13001-970 Campinas, SP, Brazil.
Plant Sci. 2006 Sep;171(3):300-7. doi: 10.1016/j.plantsci.2006.03.008. Epub 2006 Apr 18.
Drought is a major constraint for the production of common bean (Phaseolus vulgaris L.). To identify molecular responses to water deficit, we performed a differential display RT-PCR (DDRT) analysis using roots of bean plants grown aeroponically and submitted to dehydration. This allowed us to visualise 1200 DDRT bands, 8.7% of which showed a clear regulation by dehydration, and to clone 42 cDNAs, called PvD1 to PvD42. Among them, 20 early-dehydration-responsive cDNAs were selected by reverse northern that were induced or repressed before detectable water status changes and induction of ABA-regulated genes. Northern analysis for 16 PvD clones confirmed these early regulations and allowed us to identify four late dehydration-responsive genes. Their putative involvement in signalling, protein turn-over and translocation, chaperones as well as root growth modulations in response to water stress is discussed.
干旱是普通菜豆（Phaseolus vulgaris L.）生产的主要限制因素。为了鉴定对水分亏缺的分子反应，我们使用在空气培条件下生长的菜豆植株的根进行差异显示 RT-PCR（DDRT）分析，并进行了脱水处理。这使我们能够观察到 1200 个 DDRT 带，其中 8.7%的带受脱水的明显调节，并克隆了 42 个 cDNA，称为 PvD1 到 PvD42。其中，通过反向Northern 选择了 20 个早期脱水反应 cDNA，这些 cDNA 在可检测到的水分状态变化和 ABA 调节基因诱导之前被诱导或抑制。对 16 个 PvD 克隆的 Northern 分析证实了这些早期的调节，并使我们能够鉴定出 4 个晚期脱水反应基因。讨论了它们在信号转导、蛋白质周转和易位、伴侣蛋白以及根生长对水分胁迫的调节中的可能参与。
