Yin Guo-zhi, Tu Kang-sheng, Han Shao-shan, Wang Jun, Liu Qing-guang, Yao Ying-min
Department of Hepatobiliary Surgery, Xian Jiaotong University, Xian, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Sep;28(9):933-6.
To investigate the effect of nimesulide on cell apoptosis and possible mechanism in human hepatocellular carcinoma SMMC-7721 cells.
SMMC-7721 cells were treated with nimesulide at different concentrations. Cell viability was assessed by MTT assay. Cell apoptosis rate was determined with flow cytometry. The cleavage activity of PARP and caspase-9 and the expression of HSP70 were evaluated using RT-PCR and Western blotting. The influence of HSP70 on cell apoptosis was observed using RNA interference silencing HSP70 expression.
Nimesulide significantly inhibited cell growth in SMMC-7721 cells in a time- and concentration-dependent manner, and induced cell apoptosis in a concentration-dependent manner. Moreover, nimesulide promoted the cleavage of caspase-9 and PARP and inhibited the mRNA and protein expression of HSP70. Through the specific inhibition on HSP70 gene with siRNA, cell apoptosis increased, and the apoptosis was enhanced by the cleavage activity of caspase-9 and PARP.
Nimesulide could inhibit cell growth and induce apoptosis in human hepatocellular carcinoma SMMC-7721 cells via the downregulation of HSP70.
探讨尼美舒利对人肝癌SMMC - 7721细胞凋亡的影响及其可能机制。
用不同浓度的尼美舒利处理SMMC - 7721细胞。采用MTT法评估细胞活力。用流式细胞术测定细胞凋亡率。运用RT - PCR和蛋白质印迹法评估PARP和caspase - 9的切割活性以及HSP70的表达。通过RNA干扰沉默HSP70表达，观察HSP70对细胞凋亡的影响。
尼美舒利以时间和浓度依赖性方式显著抑制SMMC - 7721细胞的生长，并以浓度依赖性方式诱导细胞凋亡。此外，尼美舒利促进caspase - 9和PARP的切割，并抑制HSP70的mRNA和蛋白质表达。通过siRNA对HSP70基因的特异性抑制，细胞凋亡增加，且caspase - 9和PARP的切割活性增强了这种凋亡。
尼美舒利可通过下调HSP70抑制人肝癌SMMC - 7721细胞的生长并诱导其凋亡。
