Department of Medicinal Chemistry, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC.
Food Chem. 2012 Dec 15;135(4):2119-27. doi: 10.1016/j.foodchem.2012.07.041. Epub 2012 Jul 15.
Protective effects of sweet orange (Citrus sinensis) peel and their bioactive compounds on oxidative stress were investigated. According to HPLC-DAD and HPLC-MS/MS analysis, hesperidin (HD), hesperetin (HT), nobiletin (NT), and tangeretin (TT) were present in water extracts of sweet orange peel (WESP). The cytotoxic effect in 0.2mM t-BHP-induced HepG2 cells was inhibited by WESP and their bioactive compounds. The protective effect of WESP and their bioactive compounds in 0.2mM t-BHP-induced HepG2 cells may be associated with positive regulation of GSH levels and antioxidant enzymes, decrease in ROS formation and TBARS generation, increase in the mitochondria membrane potential and Bcl-2/Bax ratio, as well as decrease in caspase-3 activation. Overall, WESP displayed a significant cytoprotective effect against oxidative stress, which may be most likely because of the phenolics-related bioactive compounds in WESP, leading to maintenance of the normal redox status of cells.
研究了甜橙（Citrus sinensis）皮及其生物活性化合物对氧化应激的保护作用。根据 HPLC-DAD 和 HPLC-MS/MS 分析，在甜橙皮的水提取物（WESP）中存在柚皮苷（HD）、橙皮苷（HT）、川陈皮素（NT）和桔皮素（TT）。WESP 和其生物活性化合物抑制了 0.2mM t-BHP 诱导的 HepG2 细胞的细胞毒性作用。WESP 和其生物活性化合物在 0.2mM t-BHP 诱导的 HepG2 细胞中的保护作用可能与 GSH 水平和抗氧化酶的正调控、ROS 形成和 TBARS 生成的减少、线粒体膜电位和 Bcl-2/Bax 比值的增加以及 caspase-3 激活的减少有关。总的来说，WESP 对氧化应激显示出显著的细胞保护作用，这可能主要归因于 WESP 中与酚类相关的生物活性化合物，从而维持细胞的正常氧化还原状态。
