甜橙（Citrus sinensis）皮及其生物活性化合物对氧化应激的保护作用。

Protective effects of sweet orange (Citrus sinensis) peel and their bioactive compounds on oxidative stress.

机构信息

Department of Medicinal Chemistry, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC.

出版信息

Food Chem. 2012 Dec 15;135(4):2119-27. doi: 10.1016/j.foodchem.2012.07.041. Epub 2012 Jul 15.

DOI:10.1016/j.foodchem.2012.07.041

PMID:22980779

Abstract

Protective effects of sweet orange (Citrus sinensis) peel and their bioactive compounds on oxidative stress were investigated. According to HPLC-DAD and HPLC-MS/MS analysis, hesperidin (HD), hesperetin (HT), nobiletin (NT), and tangeretin (TT) were present in water extracts of sweet orange peel (WESP). The cytotoxic effect in 0.2mM t-BHP-induced HepG2 cells was inhibited by WESP and their bioactive compounds. The protective effect of WESP and their bioactive compounds in 0.2mM t-BHP-induced HepG2 cells may be associated with positive regulation of GSH levels and antioxidant enzymes, decrease in ROS formation and TBARS generation, increase in the mitochondria membrane potential and Bcl-2/Bax ratio, as well as decrease in caspase-3 activation. Overall, WESP displayed a significant cytoprotective effect against oxidative stress, which may be most likely because of the phenolics-related bioactive compounds in WESP, leading to maintenance of the normal redox status of cells.

摘要

研究了甜橙（Citrus sinensis）皮及其生物活性化合物对氧化应激的保护作用。根据 HPLC-DAD 和 HPLC-MS/MS 分析，在甜橙皮的水提取物（WESP）中存在柚皮苷（HD）、橙皮苷（HT）、川陈皮素（NT）和桔皮素（TT）。WESP 和其生物活性化合物抑制了 0.2mM t-BHP 诱导的 HepG2 细胞的细胞毒性作用。WESP 和其生物活性化合物在 0.2mM t-BHP 诱导的 HepG2 细胞中的保护作用可能与 GSH 水平和抗氧化酶的正调控、ROS 形成和 TBARS 生成的减少、线粒体膜电位和 Bcl-2/Bax 比值的增加以及 caspase-3 激活的减少有关。总的来说，WESP 对氧化应激显示出显著的细胞保护作用，这可能主要归因于 WESP 中与酚类相关的生物活性化合物，从而维持细胞的正常氧化还原状态。